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Biotecnologia de Dunaliella salina para produção de B caroteno
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Abstract(s)
O presente trabalho teve como objectivo a optimização do processo produtivo de (3 caroteno pela
microalga Dunaliella salina, numa estratégia de produção em duas fases. Na primeira fase do processo,
fase de optimização da produção de biomassa num sistema de cultivo em semi-contínuo, foram obtidas
produtividades celulares de 170 000 cel/ml/dia para condições de final de Inverno e 470 000 cel/ml/dia
em condições de Verão. Os valores de nitrato residual obtidos nas culturas da primeira fase. foram
conpatíveis com a utilização directa das culturas como inoculo na segunda. Na fase de carotenogénese.
realizada num sistema de "feed-batch", foram obtidas^produtividades de (3 caroteno de 477 mg/nr/dia
para condições de final de Inverno e 621 mg/nr/dia em condições de Verão, o controlo da
concentração de nitrato nas culturas mostrou-se eficaz na indução de síntese do pigmento e o teor em (3
caroteno e clorofila das culturas da primeira fase, mostrou ser um factor preponderante à obtenção de
culturas de elevada relação (3 caroteno/clorofila na segunda.
The present work is related with the optimization process for p carotene production from the microalgae Dunaliella salina in a two phases process. In the first phase, the biomass production optimization phase, the cultures were grow under a semi-continuos system and had reach cellular produclivities of 170 000 cel/ml/day in late Winter weather conditions and 470 000 cel/ml/day m Summer conditions. The nitrate values under the first phase were low enough for a direct utihzation ol those cultures as inoculum for the second one. In the carotenogenic phase, in a feed-batch system. the cultures had reach (3 carotene productivities of 477 mg/nr/day in late Winter conditions and 621 mg/nr/day in Summer, the culture nitrate concentration control had show good results in the pigment production induction and the cellular content of P carotene and chlorophyll in the hrsl phase cultures appears as a extremely important factor in the maximization of the p carotene/chlorophyll relation at the end ofthe second one.
The present work is related with the optimization process for p carotene production from the microalgae Dunaliella salina in a two phases process. In the first phase, the biomass production optimization phase, the cultures were grow under a semi-continuos system and had reach cellular produclivities of 170 000 cel/ml/day in late Winter weather conditions and 470 000 cel/ml/day m Summer conditions. The nitrate values under the first phase were low enough for a direct utihzation ol those cultures as inoculum for the second one. In the carotenogenic phase, in a feed-batch system. the cultures had reach (3 carotene productivities of 477 mg/nr/day in late Winter conditions and 621 mg/nr/day in Summer, the culture nitrate concentration control had show good results in the pigment production induction and the cellular content of P carotene and chlorophyll in the hrsl phase cultures appears as a extremely important factor in the maximization of the p carotene/chlorophyll relation at the end ofthe second one.
Description
Dissertação de mest. em Aquacultura, Unidade de Ciências e Tecnologia dos Recursos Aquáticos, Univ. do Algarve, 1997
Keywords
Aquacultura Dunaliella salina Microalgas