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Regulation of bicarbonate secretion in marine fish intestine by the calcium-sensing receptor

2018-04Journal article Open Access
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dc.contributor.authorGregorio, Silvia
dc.contributor.authorFuentes, Juan
dc.date.accessioned2018-12-07T14:52:25Z
dc.date.available2018-12-07T14:52:25Z
dc.date.issued2018-04
dc.description.abstractIn marine fish, high epithelial intestinal HCO3- secretion generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. The present study was designed to expose the putative role for calcium and the calcium-sensing receptor (CaSR) in the regulation of HCO3- secretion in the intestine of the sea bream (Sparus aurata L.). Effects on the expression of the CaSR in the intestine were evaluated by qPCR and an increase was observed in the anterior intestine in fed fish compared with unfed fish and with different regions of intestine. CaSR expression reflected intestinal fluid calcium concentration. In addition, anterior intestine tissue was mounted in Ussing chambers to test the putative regulation of HCO3- secretion in vitro using the anterior intestine. HCO3- secretion was sensitive to varying calcium levels in luminal saline and to calcimimetic compounds known to activate/block the CaSR i.e., R 568 and NPS-2143. Subsequent experiments were performed in intestinal sacs to measure water absorption and the sensitivity of water absorption to varying luminal levels of calcium and calcimimetics were exposed as well. It appears, that CaSR mediates HCO3- secretion and water absorption in marine fish as shown by responsiveness to calcium levels and calcimimetic compounds.
dc.description.sponsorshipEuropean Social Funds through the Portuguese National Science Foundation (FCT) [SFRH/BD/113363/2015, PTDC/MAR-BIO/3034/2014]; Portuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2013]; Ministry of Science and Higher Education
dc.identifier.doi10.3390/ijms19041072
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/11075
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationRegulation of bicarbonate transporter cascade in the intestine of marine fish.
dc.subjectBream Sparus-Aurata
dc.subjectHormone-Related Protein
dc.subjectExtracellular Ca2+-Sensing Receptor
dc.subjectSoluble Adenylyl-Cyclase
dc.subjectSea Bream
dc.subjectWater-Absorption
dc.subjectTeleost Fish
dc.subjectParathyroid-Hormone
dc.subjectHco3-Secretion
dc.subjectRainbow-Trout
dc.titleRegulation of bicarbonate secretion in marine fish intestine by the calcium-sensing receptor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRegulation of bicarbonate transporter cascade in the intestine of marine fish.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F113363%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3034%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.issue4
oaire.citation.startPage1072
oaire.citation.titleInternational Journal of Molecular Sciences
oaire.citation.volume19
oaire.fundingStreamOE
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameGregorio
person.familyNameFuentes
person.givenNameSilvia
person.givenNameJuan
person.identifier1579837
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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