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Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

2014Journal article Restricted access
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dc.contributor.authorMartins, I.
dc.contributor.authorMartins, F.
dc.contributor.authorBelo, H.
dc.contributor.authorVaz, M.
dc.contributor.authorCarvalho, M.
dc.contributor.authorCravador, A.
dc.contributor.authorChoupina, A.
dc.date.accessioned2015-06-15T09:15:51Z
dc.date.available2015-06-15T09:15:51Z
dc.date.issued2014
dc.description.abstractOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamomi. In this work we report the sequencing and characterization of a class of GIPs secreted by Phytophthora cinnamomi. The gip gene from P. cinnamomi has a 937 bp ORF encoding a putative peptide of 312 deduced amino acids. The expression of this gene was studied during growth in different carbon sources (glucose, cellulose and sawdust), by RT-qPCR and its level of expression was evaluated at five time points. The highest expression of gip gene occurred in sawdust at 8 h of induction. In vivo infection of C. sativa revealed an increase in gip expression from 12 to 24 h. At 36 h its expression decreased suggesting that a compensatory mechanism must occur in plant.
dc.identifier.doihttps://dx.doi.org/10.1007/s11033-014-3101-1
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.otherAUT: ACR00659;
dc.identifier.urihttp://hdl.handle.net/10400.1/6130
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-009-8NH
dc.titleCloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT
oaire.citation.endPage2462
oaire.citation.startPage2453
oaire.citation.titleMolecular Biology Reports
oaire.citation.volume41
oaire.fundingStream3599-PPCDT
person.familyNameCravador
person.givenNameAlfredo
person.identifier.orcid0000-0002-9831-9815
person.identifier.ridK-7247-2012
person.identifier.scopus-author-id6602537257
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isAuthorOfPublication.latestForDiscovery8c84a9e6-6b7f-4b50-93a2-152c85901722
relation.isProjectOfPublication9b7d24b8-1f01-4de4-ad2e-590347acee67
relation.isProjectOfPublication.latestForDiscovery9b7d24b8-1f01-4de4-ad2e-590347acee67

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