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Marine sponge and octocoral-associated bacteria show versatile secondary metabolite biosynthesis potential and antimicrobial activities against human pathogens

2022Journal article Open access
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dc.contributor.authorAlmeida, João F.
dc.contributor.authorMarques, Matilde
dc.contributor.authorOliveira, Vanessa
dc.contributor.authorEgas, Conceição
dc.contributor.authorMil-Homens, Dalila
dc.contributor.authorViana, Romeu
dc.contributor.authorCleary, Daniel F. R.
dc.contributor.authorHuang, Yusheng M.
dc.contributor.authorFialho, Arsénio M.
dc.contributor.authorTeixeira, Miguel C.
dc.contributor.authorGomes, Newton C. M.
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorKeller-Costa, Tina
dc.date.accessioned2023-02-27T11:40:18Z
dc.date.available2023-02-27T11:40:18Z
dc.date.issued2022
dc.description.abstractMarine microbiomes are prolific sources of bioactive natural products of potential pharmaceutical value. This study inspected two culture collections comprising 919 host-associated marine bacteria belonging to 55 genera and several thus-far unclassified lineages to identify isolates with potentially rich secondary metabolism and antimicrobial activities. Seventy representative isolates had their genomes mined for secondary metabolite biosynthetic gene clusters (SM-BGCs) and were screened for antimicrobial activities against four pathogenic bacteria and five pathogenic Candida strains. In total, 466 SM-BGCs were identified, with antimicrobial peptide- and polyketide synthase-related SM-BGCs being frequently detected. Only 38 SM-BGCs had similarities greater than 70% to SM-BGCs encoding known compounds, highlighting the potential biosynthetic novelty encoded by these genomes. Cross-streak assays showed that 33 of the 70 genome-sequenced isolates were active against at least one Candida species, while 44 isolates showed activity against at least one bacterial pathogen. Taxon-specific differences in antimicrobial activity among isolates suggested distinct molecules involved in antagonism against bacterial versus Candida pathogens. The here reported culture collections and genome-sequenced isolates constitute a valuable resource of understudied marine bacteria displaying antimicrobial activities and potential for the biosynthesis of novel secondary metabolites, holding promise for a future sustainable production of marine drug leads.pt_PT
dc.description.sponsorshipPOCI-01-0145-FEDER-016531
dc.description.sponsorshipLA/P/0140/2020
dc.description.sponsorshipLA/P/0094/2020
dc.description.sponsorshipLA/P/0058/2020
dc.description.sponsorshipPOCI-01-0145-FEDER-022184
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/md21010034pt_PT
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/19138
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCEECIND/ 00788/2017pt_PT
dc.relationAssessing the ECOlogical functions and potential bioTECHnological applications of plasmid assemblages from microbial symbionts of marine SPONGEs
dc.relationInstitute for Bioengineering and Biosciences
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioprospectionpt_PT
dc.subjectBiosynthetic gene clusterspt_PT
dc.subjectBlue biotechnologypt_PT
dc.subjectCulture collectionspt_PT
dc.subjectGenomicspt_PT
dc.subjectMarine bacteriapt_PT
dc.titleMarine sponge and octocoral-associated bacteria show versatile secondary metabolite biosynthesis potential and antimicrobial activities against human pathogenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssessing the ECOlogical functions and potential bioTECHnological applications of plasmid assemblages from microbial symbionts of marine SPONGEs
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIA-MIC%2F6473%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04539%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage34pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume21pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Silva Costa
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery4495127c-16f2-4231-9fc1-b471c661036b
relation.isProjectOfPublication154b1e4b-a589-4729-850a-98faa7d1a01b
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