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Cells isolated from regenerating caudal fin of Sparus aurata can differentiate into distinct bone cell lineages

2020-06Journal article Restricted
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dc.contributor.authorVijayakumar, Parameswaran
dc.contributor.authorCardeira, João
dc.contributor.authorLaizé, Vincent
dc.contributor.authorJ. Gavaia, Paulo
dc.contributor.authorCancela, M. Leonor
dc.date.accessioned2021-06-24T11:35:54Z
dc.date.available2021-06-24T11:35:54Z
dc.date.issued2020-06
dc.description.abstractTeleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest that the blastema is comprised of lineage-restricted dedifferentiated cells. To investigate the differentiation capacity of regenerating fin-derived cells, primary cultures were initiated from the explants of 2-days post-amputation (dpa) regenerates of juvenile gilthead seabream (Sparus aurata). These cells were subcultured for over 30 passages and were named as BSa2. After 10 passages they were characterized for their ability to differentiate towards different bone cell lineages and mineralize their extracellular matrix, through immunocytochemistry, histology, and RT-PCR. Exogenous DNA was efficiently delivered into these cells by nucleofection. Assessment of lineage-specific markers revealed that BSa2 cells were capable of osteo/chondroblastic differentiation. BSa2 cells were also found to be capable of osteoclastic differentiation, as demonstrated through TRAP-specific staining and pit resorption assay. Here, we describe the development of the first successful cell line viz., BSa2, from S. aurata 2-dpa regenerating caudal fins, which has the ability of multilineage differentiation and is capable of in vitro mineralization. The availability of such in vitro cell systems has the potential to stimulate research on the mechanisms of cell differentiation during fin regeneration and provide new insights into the mechanisms of bone formation.
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT)Portuguese Foundation for Science and Technology [PTDC/MAR/105313/2008, UID/Multi/04326/2019]
dc.description.sponsorshipEuropean Community (EC)European CommissionEuropean Commission Joint Research Centre [FP7/227799]
dc.description.sponsorshipFCTPortuguese Foundation for Science and TechnologyEuropean Commission [SFRH/BPD/39189/2007]
dc.identifier.doi10.1007/s10126-019-09937-3
dc.identifier.issn1436-2228
dc.identifier.urihttp://hdl.handle.net/10400.1/16571
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationDevelopment and characterization of zebrafish stem cells: implications for transgenesis - FishCell
dc.relationCentre of Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBlastema
dc.subjectCaudal fin
dc.subjectRegeneration
dc.subjectCell line
dc.subjectGilthead seabream
dc.subjectDifferentiation
dc.subject.otherBiotechnology & Applied Microbiology; Marine & Freshwater Biology
dc.titleCells isolated from regenerating caudal fin of Sparus aurata can differentiate into distinct bone cell lineages
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment and characterization of zebrafish stem cells: implications for transgenesis - FishCell
oaire.awardTitleCentre of Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F39189%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F105313%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.citation.endPage347
oaire.citation.issue3
oaire.citation.startPage333
oaire.citation.titleMarine Biotechnology
oaire.citation.volume22
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVijayakumar
person.familyNameCardeira da Silva
person.familyNameLaizé
person.familyNameGavaia
person.familyNameCancela
person.givenNameParameswaran
person.givenNameJoão
person.givenNameVincent
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-idDC15-86F7-3AAC
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-1345-586X
person.identifier.orcid0000-0003-3722-8810
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridE-5298-2017
person.identifier.ridB-4463-2008
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id8973850300
person.identifier.scopus-author-id55224020100
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6507104377
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
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