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Adaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model

2013-06Journal article Open access
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dc.contributor.authorSchrama, Denise
dc.contributor.authorHelliwell, N.
dc.contributor.authorNeto, L.
dc.contributor.authorFaleiro, Maria Leonor
dc.date.accessioned2018-12-07T14:53:32Z
dc.date.available2018-12-07T14:53:32Z
dc.date.issued2013-06
dc.description.abstractThe aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of Listeria monocytogenes strains isolated from cheese and dairy processing environment using the Galleria mellonella model. Four L.monocytogenes strains were exposed to a cheese-based medium in conditions of induction of an acid tolerance response and osmotolerance response (pH 5 center dot 5 and 3 center dot 5% w/v NaCl) and injected in G.mellonella insects. The survival of insects and the L.monocytogenes growth kinetics in insects were evaluated. The gene expression of hly, actA and inlA genes was determined by real-time PCR. The adapted cells of two dairy strains showed reduced insect mortality (P<0 center dot 05) in comparison with nonadapted cells. Listeria monocytogenes Scott A was the least virulent, whereas the cheese isolate C882 caused the highest insect mortality, and no differences (0 center dot 05) was found between adapted and nonadapted cells. The gene expression results evidenced an overexpression of virulence genes in cheese-based medium, but not in simulated insect-induced conditions. Our results suggest that adaptation to low pH and salt in a cheese-based medium can affect the virulence of L.monocytogenes, but this effect is strain dependent. Significance and Impact of the Study In this study, the impact of adaptation to low pH and salt in a cheese-based medium on L.monocytogenes virulence was tested using the Wax Moth G.mellonella model. This model allowed the differentiation of the virulence potential between the L.monocytogenes strains. The effect of adaptation on virulence is strain dependent. The G.mellonella model revealed to be a prompt method to test food-related factors on L.monocytogenes virulence.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia (FCT); IBB/CBME, LA, FEDER/
dc.description.sponsorshipThe authors are grateful for the support of Fundação para a Ciência e Tecnologia (FCT) and IBB/CBME, LA, FEDER/
dc.identifier.doi10.1111/lam.12064
dc.identifier.issn0266-8254
dc.identifier.urihttp://hdl.handle.net/10400.1/11561
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley-Blackwell
dc.publisherWiley
dc.subjectAcid tolerance response
dc.subjectStress
dc.subjectSurvive
dc.subjectCells
dc.subjectCycle
dc.subjectMilk
dc.subjectSalt
dc.titleAdaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage427
oaire.citation.issue6
oaire.citation.startPage421
oaire.citation.titleLetters in Applied Microbiology
oaire.citation.volume56
person.familyNameSchrama
person.familyNameFaleiro
person.givenNameDenise
person.givenNameMaria Leonor
person.identifier159154
person.identifier252666
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.ciencia-id281D-5E01-F09A
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0002-3878-6948
person.identifier.ridH-6511-2016
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id6507927018
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication00f4fa6e-b467-4d5c-83f2-a454f7b4bc71
relation.isAuthorOfPublication5361e29a-3b79-4ce8-9f87-0c667d60588d
relation.isAuthorOfPublication.latestForDiscovery5361e29a-3b79-4ce8-9f87-0c667d60588d

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