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Electrochemical determination of natural drug colchicine in pharmaceuticals and human serum sample and its Interaction with DNA

2017-10Journal article Restricted access
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dc.contributor.authorStankovic, Dalibor M.
dc.contributor.authorSvorc, Lubomir
dc.contributor.authorMariano, José
dc.contributor.authorOrtner, Astrid
dc.contributor.authorKalcher, Kurt
dc.date.accessioned2019-11-20T15:07:18Z
dc.date.available2019-11-20T15:07:18Z
dc.date.issued2017-10
dc.description.abstractColchicine (COLC) is a natural toxic product and secondary metabolite most commonly used to treat gout. In this study, its electrochemical behavior and determination was investigated by employing modification-free boron-doped diamond electrode (BDDE). Besides, its interaction with DNA was monitored using electrochemical methods. It was found that oxidation of this compound proceeds in two steps, where first sharp and well defined oxidation peak occurs at potential of around 1.19V, and second one at around 1.37V, in Britton-Robinson buffer solution at pH7.5. Wide dynamic range from 1 to 100 mu M was obtained with a detection limit (3 sigma(intercept)/slope) of a 0.26 mu M, based on the evaluation of first oxidation peak using differential pulse voltammetry. The proposed method was also found to be suitable for monitoring interaction of this drug with DNA as important segment for medical use. Concerning the validation, the analytical procedure shows excellent selectivity and sensitivity toward COLC detection and after method development it was successfully used for its quantification in pharmaceutical preparation and human serum sample, with satisfactory recovery. Obviously, this approach can be promising replacement for time-consuming and expensive separation methods.
dc.description.sponsorshipMagbiovin project [621375]
dc.description.sponsorshipMinistry of Education, Science and Technology, the Republic of Serbia [OI 172030]
dc.description.sponsorshipGrant Agency of the Slovak Republic [1/0489/16]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1002/elan.201700233
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.urihttp://hdl.handle.net/10400.1/12970
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley-V C H Verlag Gmbh
dc.relationStrengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials
dc.subjectDoped diamond electrode
dc.subjectGlassy-carbon electrode
dc.subjectVoltammetric determination
dc.subjectSensitive determination
dc.subjectGraphite
dc.subjectProducts
dc.subjectCaffeine
dc.subjectSensor
dc.subjectAcid
dc.titleElectrochemical determination of natural drug colchicine in pharmaceuticals and human serum sample and its Interaction with DNA
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/621375/EU
oaire.citation.endPage2281
oaire.citation.issue10
oaire.citation.startPage2276
oaire.citation.titleElectroanalysis
oaire.citation.volume29
oaire.fundingStreamFP7
person.familyNameMariano
person.givenNameJosé
person.identifier.ciencia-idDE19-7D9D-00D0
person.identifier.orcid0000-0002-8480-617X
person.identifier.ridO-5252-2018
person.identifier.scopus-author-id12647832000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication16ee0cfc-3dd4-40fa-8401-b435aa41ef7d
relation.isAuthorOfPublication.latestForDiscovery16ee0cfc-3dd4-40fa-8401-b435aa41ef7d
relation.isProjectOfPublicationcf234961-2f25-4cc3-8553-802ca7fc5abc
relation.isProjectOfPublication.latestForDiscoverycf234961-2f25-4cc3-8553-802ca7fc5abc

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