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Cloning of the cDNA for the putative calcium-sensing receptor and its tissue distribution in sea bream (Sparus aurata)

2002Journal article Restricted
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dc.contributor.authorFlanagan, J. A.
dc.contributor.authorBendell, L. A.
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorClark, M. S.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorBrown, B. L.
dc.contributor.authorIngleton, P. M.
dc.date.accessioned2014-05-13T11:01:52Z
dc.date.available2014-05-13T11:01:52Z
dc.date.issued2002
dc.date.updated2014-05-09T09:47:21Z
dc.description.abstractThe cDNA for the calcium-sensing receptor (CaSR) gene has been cloned from the marine teleost Sparus aurata, the sea bream. The isolated clones were 3.3 kb long with an open reading frame of 2820 bp, a 50 UTR of 240 bp, and 30 UTR of 248 bp. The gene codes for a mature peptide of 940 amino acids which has three principal domains; the extracellular region is more than half the total protein, there is a seven-transmembrane domain, and there is a short intracellular domain. There is considerable sequence identity, 91%, shared between the CaSR of sea bream and puffer fish but overall similarities with mammalian CaSR peptides vary between 44% for rat and mouse and 48% with human CaSR. Nevertheless, the 18 cysteine residues of the extracellular domain are present in all sequences so far analysed of which 9 form a cysteine-rich region in sea bream similar to mammalian CaSR. The distribution of CaSR in sea bream tissues detected by in situ hybridisation showed gene expression in epithelia associated with ion transport or ion regulation including the hind gut, chloride cells of the gills, operculum, gall bladder, pituitary adenohypophysis, and coronet cells of the saccus vasculosus; this distribution was confirmed by RT-PCR. By in situ hybridisation, CaSR gene expression was also present in olfactory nerves and leucocytes.por
dc.identifier.citationFlanagan, J.A; Bendell, L.A; Guerreiro, P.M; Clark, M.S; Power, D.M; Canario, A.V.M; Brown, B.L; Ingleton, P.M. Cloning of the cDNA for the putative calcium-sensing receptor and its tissue distribution in sea bream (Sparus aurata), General and Comparative Endocrinology, 127, 2, 117-127, 2002.por
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3897
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAcademic Presspor
dc.subjectCalcium-sensing receptorpor
dc.subjectMarine teleostpor
dc.subjectcDNApor
dc.titleCloning of the cDNA for the putative calcium-sensing receptor and its tissue distribution in sea bream (Sparus aurata)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage127por
oaire.citation.issue2por
oaire.citation.startPage117por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume127por
person.familyNameGuerreiro da Costa Guerreiro
person.familyNamePower
person.familyNameCanario
person.givenNamePedro Miguel
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryd0a8a148-98e7-4899-8fb0-0b8afa91e4a4

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