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Holothurians have a reduced GPCR and odorant receptor-like repertoire compared to other echinoderms

2020Journal article Open access
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dc.contributor.authorMarquet, Nathalie
dc.contributor.authorCardoso, João CR
dc.contributor.authorLouro, Bruno
dc.contributor.authorFernandes, Stefan
dc.contributor.authorSilva, Sandra
dc.contributor.authorCanario, Adelino
dc.date.accessioned2020-09-25T08:58:04Z
dc.date.available2020-09-25T08:58:04Z
dc.date.issued2020
dc.description.abstractSea cucumbers lack vision and rely on chemical sensing to reproduce and survive. However, how they recognize and respond to environmental cues remains unknown. Possible candidates are the odorant receptors (ORs), a diverse family of G protein-coupled receptors (GPCRs) involved in olfaction. The present study aimed at characterizing the chemosensory GPCRs in sea cucumbers. At least 246 distinct GPCRs, of which ca. 20% putative ORs, were found in a transcriptome assembly of putative chemosensory (tentacles, oral cavity, calcareous ring, and papillae/tegument) and reproductive (ovary and testis) tissues from Holothuria arguinensis (57 ORs) and in the Apostichopus japonicus genome (79 ORs). The sea cucumber ORs clustered with those of sea urchin and starfish into four main clades of gene expansions sharing a common ancestor and evolving under purifying selection. However, the sea cucumber ORs repertoire was the smallest among the echinoderms and the olfactory receptor signature motif LxxPxYxxxxxLxxxDxxxxxxxxP was better conserved in cluster OR-l1 which also had more members. ORs were expressed in tentacles, oral cavity, calcareous ring, and papillae/tegument, supporting their potential role in chemosensing. This study is the first comprehensive survey of chemosensory GPCRs in sea cucumbers, and provides the molecular basis to understand how they communicate.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology: UIDB/04326/2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-020-60167-3pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/14744
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationTRANSCRIPTOMIC RESPONSES TO THERMAL AND OSMOREGULATORY CHALLENGES OF ANTARCTIC NOTOTHENIOID FISH: AN INSIGHT INTO RESPONSES TO GLOBAL CHANGE AND GENOME EVOLUTION
dc.relationSTUDY OF REPRODUCTIVE BIOLOGY AND CHEMICAL COMMUNICATION OF SEA CUCUMBERS HOLOTHURIA ARGUINENSIS AND H. MAMMATA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectProtein-couple receptorspt_PT
dc.subjectPutative pheromone receptorspt_PT
dc.titleHolothurians have a reduced GPCR and odorant receptor-like repertoire compared to other echinodermspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTRANSCRIPTOMIC RESPONSES TO THERMAL AND OSMOREGULATORY CHALLENGES OF ANTARCTIC NOTOTHENIOID FISH: AN INSIGHT INTO RESPONSES TO GLOBAL CHANGE AND GENOME EVOLUTION
oaire.awardTitleSTUDY OF REPRODUCTIVE BIOLOGY AND CHEMICAL COMMUNICATION OF SEA CUCUMBERS HOLOTHURIA ARGUINENSIS AND H. MAMMATA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89889%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F90761%2F2012/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage3348pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume10pt_PT
person.familyNameMarquet
person.familyNameCardoso
person.familyNameLouro
person.familyNameSilva
person.familyNameCanario
person.givenNameNathalie
person.givenNameJoão
person.givenNameBruno
person.givenNameSandra
person.givenNameAdelino
person.identifierN-6804-2013
person.identifier14332
person.identifier143624
person.identifier.ciencia-id5613-BBCD-0EFE
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id9A10-9756-78D0
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-3718-4478
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0001-8164-581X
person.identifier.orcid0000-0002-6692-3620
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridM-4151-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id57193647721
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id16028940200
person.identifier.scopus-author-id56568523700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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