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Evaluation of parental and transgenerational effects of clotrimazole in Daphnia magna — A multi-parametric approach

2022Journal article Restricted access
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dc.contributor.authorFerreira, David
dc.contributor.authorAlkimin, G. D.
dc.contributor.authorNeves, Bruna
dc.contributor.authorConde, Tiago
dc.contributor.authorDomingues, M. Rosário
dc.contributor.authorNunes, Bruno
dc.date.accessioned2022-10-11T08:41:13Z
dc.date.available2022-10-11T08:41:13Z
dc.date.issued2022
dc.description.abstractAzole antifungals inhibit the cytochrome P450 complex, decreasing the production of ergosterol in fungi, and compromising the biosynthesis of ecdysteroids in crustaceans, which are hormones regulating reproduction and ecdy-sis. The azole antifungal clotrimazole (CLO) raises environmental concerns due its toxicity. This work evaluated the effects on the number of moults, feeding rate, growth, reproduction, transgenerational reproductive effects on two dif-ferent generations (F0, parental generation; and F1, organisms born from F0), and energetic balance in Daphnia magna. Neonates (<24 h) were exposed to sublethal concentrations (0, 2.7, and 3.4 mg/L) of CLO, to assess its effects on the moulting process. Neonates were also exposed to environmentally realistic concentrations of CLO (0, 30,150, 750, and 3750 ng/L) for 24 and 96 h, to assess adverse effects on their feeding behaviour. Effects on energy reserves (fatty acids, glycogen, and protein levels) were also measured in animals exposed to CLO. A reproduction test was carried out to evaluate the amount and size of neonates from F0 and F1 generations. CLO exposure decreased the number of moults, and the size of organisms, but did not alter the feeding pattern of 5 days old individuals. However, neonates (<24 h) exposed to CLO had a significant decrease in their feeding pattern. CLO decreased the fatty acids content in exposed animals, but did not change glycogen and protein. CLO also decreased the size of adult daphnids from the third brood, born from animals exposed in F0; in F1 animals, the size of neonates from the third brood was decreased. This study evidenced the toxic effects caused by CLO on growth, feeding and reproduction of D. magna. Nevertheless, it is not pos-sible to conclude whether the effects are due to the inhibition of cytochrome P450 enzymes, or to unspecific effects caused by general toxic stress and decreased nutrition.pt_PT
dc.description.sponsorshipPOCI-01-0145-FEDER-029203
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.scitotenv.2022.154677pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/18360
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Environmental and Marine Studies
dc.relationAVOBIOMICS: Valorization of Portuguese AVOcado as a source of high added-value BIoactive lipids using OMICS-based approaches
dc.relationMiAL MicroAlgae Lipids – Exploitation of microalgae lipidome: a lipidomics strategy to bioprospect bioactive phytochemicals
dc.relationCentre for Environmental and Marine Studies
dc.subjectAzole fungicidespt_PT
dc.subjectDaphnia magnapt_PT
dc.subjectClotrimazolept_PT
dc.subjectReproductionpt_PT
dc.subjectFeedingpt_PT
dc.subjectFatty acidspt_PT
dc.titleEvaluation of parental and transgenerational effects of clotrimazole in Daphnia magna — A multi-parametric approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleAVOBIOMICS: Valorization of Portuguese AVOcado as a source of high added-value BIoactive lipids using OMICS-based approaches
oaire.awardTitleMiAL MicroAlgae Lipids – Exploitation of microalgae lipidome: a lipidomics strategy to bioprospect bioactive phytochemicals
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/2021.04602.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2020.05678.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.citation.startPage154677pt_PT
oaire.citation.titleScience of The Total Environmentpt_PT
oaire.citation.volume831pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias de Alkimin
person.givenNameGilberto
person.identifier.ciencia-id6117-E32E-461B
person.identifier.orcid0000-0002-6441-1293
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication22fbb3bf-097a-47fb-b9d9-3795c0930bc3
relation.isAuthorOfPublication.latestForDiscovery22fbb3bf-097a-47fb-b9d9-3795c0930bc3
relation.isProjectOfPublication010db565-8f84-4215-8139-a43c6816c7a0
relation.isProjectOfPublication8a303ea3-e025-4826-ae36-291c7ec5a8ec
relation.isProjectOfPublicationd34fc208-7bb9-4323-aad0-b06632acbcd3
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