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Development and validation of breeder-friendly KASPar markers for er1, a powdery mildew resistance gene in pea (Pisum sativum L.)

2017-12Journal article Restricted access
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dc.contributor.authorMa, Yu
dc.contributor.authorCoyne, Clarice J.
dc.contributor.authorMain, Dorrie
dc.contributor.authorPavan, Stefano
dc.contributor.authorSun, Suli
dc.contributor.authorZhu, Zhendong
dc.contributor.authorZong, Xuxiao
dc.contributor.authorLeitão, José
dc.contributor.authorMcGee, Rebecca J.
dc.date.accessioned2019-11-20T15:07:09Z
dc.date.available2019-11-20T15:07:09Z
dc.date.issued2017-12
dc.description.abstractPowdery mildew of pea is caused by Erysiphe pisi DC and is a serious threat to pea (Pisum sativum L.) production throughout much of the world. Development and utilization of genetic resistance to powdery mildew is considered an effective and sustainable strategy to manage this disease. One gene, er1, conferring powdery mildew resistance, was previously cloned and sequenced, and the functional markers for each resistance allele were reported. Allele-specific DNA markers are efficient and powerful tools to facilitate crop improvement and new cultivar development in breeding programs. However, extensive application of these markers is limited by gel-associated obstacles. In this study, eight breeder-friendly kompetitive allele-specific PCR (KASPar) markers were developed to overcome the problems of gel-based markers and increase the efficiency of genotypic screening. In order to identify additional pea germplasm with powdery mildew resistance, these KASPar markers were deployed and used to genotype a pea collection derived from the USDA pea single-plant (PSP) collection. Simultaneously, a phenotypic screening and a genotypic validation using the corresponding gel-based functional markers were conducted on the PSP collection. One pea accession, PI 142775, was identified by both phenotyping and genotyping to carry the allele er1-1 for powdery mildew resistance, indicating that the KASPar assay is an efficient and robust tool for breeding for powdery mildew resistance.
dc.description.sponsorshipWashington State Department of Agriculture Specialty Crop Block Grant [K1273]
dc.description.sponsorshipUSA Dry Pea and Lentil Council Research grant
dc.identifier.doi10.1007/s11032-017-0740-7
dc.identifier.issn1380-3743
dc.identifier.issn1572-9788
dc.identifier.urihttp://hdl.handle.net/10400.1/12909
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.subjectFunctional Markers
dc.subjectErysiphe-Trifolii
dc.subjectPlants
dc.subjectIdentification
dc.subjectMutations
dc.subjectSelection
dc.titleDevelopment and validation of breeder-friendly KASPar markers for er1, a powdery mildew resistance gene in pea (Pisum sativum L.)
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.startPage151
oaire.citation.titleMolecular Breeding
oaire.citation.volume37
person.familyNameLeitão
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f

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