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Rheological and microstructural features of plant culture media doped with biopolymers: Influence on the growth and physiological responses of in Vitro-Grown Shoots of Thymus lotocephalus

2021-06-17Journal article Open Access
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dc.contributor.authorCoelho, Natacha
dc.contributor.authorFilipe, Alexandra
dc.contributor.authorMedronho, Bruno
dc.contributor.authorMagalhães, Solange
dc.contributor.authorVitorino, Carla
dc.contributor.authorAlves, Luís
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorRomano, Anabela
dc.date.accessioned2021-06-25T09:52:07Z
dc.date.available2021-06-25T09:52:07Z
dc.date.issued2021-06-17
dc.date.updated2021-06-24T14:10:27Z
dc.description.abstractIn vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L<sup>−1</sup>, were tested regarding their influence on the growth and physiological responses of <i>Thymus lotocephalus</i> in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L<sup>−1</sup> significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to <i>T. lotocephalus</i>. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on <i>T. lotocephalus</i>. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L<sup>−1</sup>. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by <i>T. lotocephalus</i> shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to <i>T. lotocephalus</i> shoots growing <i>in vitro</i>, and that CMC improves shoot length.pt_PT
dc.description.sponsorshipUIDB/05183/2020, UIDB/00102/2020, PTDC/ASP-SIL/30619/2017, CEECIND/ 01014/2018, DL 57/2016/CP1361/CT0022,pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPolysaccharides 2 (2): 538-553 (2021)pt_PT
dc.identifier.doi10.3390/polysaccharides2020032pt_PT
dc.identifier.eissn2673-4176
dc.identifier.urihttp://hdl.handle.net/10400.1/16660
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCarboxymethyl cellulosept_PT
dc.subjectChitosanpt_PT
dc.subjectComplex viscositypt_PT
dc.subjectGum arabicpt_PT
dc.subjectHydroxyethyl cellulosept_PT
dc.subjectPorositpt_PT
dc.subjectScanning electron microscopypt_PT
dc.subjectShoot elongationpt_PT
dc.subjectSsurface areapt_PT
dc.titleRheological and microstructural features of plant culture media doped with biopolymers: Influence on the growth and physiological responses of in Vitro-Grown Shoots of Thymus lotocephaluspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage553pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage538pt_PT
oaire.citation.titlePolysaccharidespt_PT
oaire.citation.volume2pt_PT
person.familyNameCoelho
person.familyNameMedronho
person.familyNameGonçalves
person.familyNameRomano
person.givenNameNatacha
person.givenNameBruno
person.givenNameSandra
person.givenNameAnabela
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.ciencia-id6918-9AB3-8D22
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0003-0972-1739
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridP-5927-2014
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id9940656700
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication43b2d7ec-049a-412b-ad22-c9db64cc572c
relation.isAuthorOfPublication4c03571a-93da-4504-8acf-c74326449852
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverya8b335b2-45cc-4578-89b0-153273014280

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