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Intestinal response to ocean acidification in the European sea bass (Dicentrarchus labrax)

2020-12Journal article Restricted access
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dc.contributor.authorAlves, Alexandra
dc.contributor.authorGregorio, Silvia F.
dc.contributor.authorRuiz-Jarabo, Ignacio
dc.contributor.authorFuentes, Juan
dc.date.accessioned2021-06-18T16:25:45Z
dc.date.available2021-06-18T16:25:45Z
dc.date.issued2020-12
dc.description.abstractthe intestine of marine fishes contributes to the ocean carbon cycle producing carbonate aggregates as part of the osmoregulatory process. Therefore, this study aimed to evaluate physiological adjustments of European sea bass (Dicentrarchus labrax) intestine to a higher pCO(2) environment likely in the near future (similar to 1700 mu atm). At the whole-body level, hypercapnia for 5 weeks resulted in fish having a significantly diminished specific growth rate, condition factor and hepatosomatic index. An increase in plasma osmolality and HCO3- concentration was detected, paralleled by decreased metabolites concentrations. In the intestine, high seawater pCO(2) was without effect on ouabain-sensitive ATPase activities, while Bafilomycin A1-sensitive ATPase activity significantly decreased in the anterior intestine. Anterior and mid intestine were mounted in Ussing chambers in order to measure bioelectrical parameters and bicarbonate secretion by pH-Stat ex-vivo. Hypercapnia induced a 2.3 and 2.8-fold increase in bicarbonate secretion rates in the anterior and mid intestine, respectively. In the intestinal fluid, HCO3- concentration increased 2.2-fold, and carbonate precipitates showed a 4.4-fold increase in response to hypercapnia, paralleled by a > 3-fold increase of drinking and a > 2-fold increase of intestinal volume at any given time. At the molecular level, hypercapnia elicited higher intestinal mRNA expression levels for atp6v1b (V-ATPase B subunit), slc4a4, slc26a3, and slc26a6, both in the anterior and mid intestine. As a whole, our results show that the intestine of sea bass responds to high seawater pCO(2), a response that comes at a cost at the whole-body level with an impact in the fish specific growth rate, condition factor, and hepatosomatic index.
dc.description.sponsorshipMinistry of Science and Higher Education, Portugal
dc.description.sponsorshipEuropean Social Funds through the Portuguese National Science Foundation (FCT)[PTDC/MAR-BIO/3034/2014]
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [UID/Multi/04326/2019]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.cbpa.2020.110789
dc.identifier.issn1095-6433
dc.identifier.urihttp://hdl.handle.net/10400.1/15663
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectHCO3-secretion
dc.subjectIntestine
dc.subjectAcid-base balance
dc.subjectHypercapnia
dc.subjectMarine fish
dc.subjectSea bass
dc.subjectOcean acidification
dc.subject.otherBiochemistry & Molecular Biology
dc.subject.otherPhysiology
dc.subject.otherZoology
dc.titleIntestinal response to ocean acidification in the European sea bass (Dicentrarchus labrax)
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/141661/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154715/PT
oaire.citation.startPage110789
oaire.citation.titleComparative Biochemistry and Physiology A-Molecular & Integrative Physiology
oaire.citation.volume250
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCarvalho Alves
person.familyNameGregorio
person.familyNameRuiz-Jarabo
person.familyNameFuentes
person.givenNameAlexandra
person.givenNameSilvia
person.givenNameIgnacio
person.givenNameJuan
person.identifier1579837
person.identifier392998
person.identifier.ciencia-id8D19-0844-ACCF
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0003-1153-596X
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0002-3918-0921
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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