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In vitro antimicrobial and synergistic effect of essential oil from the red macroalgae Centroceras clavulatum (C. Agardh) Montagne with conventional antibiotics

2021-09Journal article Open Access
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dc.contributor.authorCustódio, Luísa
dc.contributor.authorNafis, Ahmed
dc.contributor.authorEl Khalloufi, Fatima
dc.contributor.authorAknaf, Asmae
dc.contributor.authorOudra, Brahim
dc.contributor.authorMarraiki, Najat
dc.contributor.authorAl-Rashed, Sarah
dc.contributor.authorElgorban, AbdallahM
dc.contributor.authorSyed, Asad
dc.contributor.authorHassani, Lahcen
dc.date.accessioned2021-09-02T15:40:11Z
dc.date.available2021-09-02T15:40:11Z
dc.date.issued2021-09
dc.description.abstractObjective: To study the chemical profile, antimicrobial properties, and synergistic effect with known antibiotics of essential oil extracted from the marine red macroalgae Centroceras clavulatum (C. Agardh) Montagne, collected in Morocco. Methods: The chemical composition of the oil was analyzed by gas chromatography-mass spectrometry. The oil was evaluated for antibacterial (Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, and Klebsiella pneumoniae), and antifungal activity (Candida albicans, Candida glabrata, Candida krusei, and Candida parapsilosis), by the disc diffusion method. The minimum inhibitory and minimum microbicidal concentrations of the oil were determined, as well as the synergistic effects of its application combined with the antibiotics ciprofloxacin and fluconazole, by the checkerboard method. Results: Thirty molecules were identified in the essential oil, comprising 96.27% of the total oil composition. Monoterpenes such as carvacrol (36.06%) were the most abundant compounds, followed by caryophyllene (14.67%), endo-borneol (9.04%), pyroterebic acid (3.23%) and caryophyllene oxide (3.13%). The oil exhibited a moderate antimicrobial activity with inhibition zone diameters ranging from 9.0 to 15.0 mm. The minimum inhibitory concentration values varied between 0.9 and 14.7 mg/mL, and Bacillus subtilis and Escherichia coli were the more sensitive bacteria with 0.9 and 1.9 mg/mL, respectively. The minimummicrobicidal concentration values ranged from 0.4 to 14.7 mg/mL. A significant synergic action was observed when the oil was applied in combination with ciprofloxacin and fluconazole, with fractional inhibitory concentration index values ranging from 0.31 to 0.50. Synergy was found in 80% of the combinations and a 2 to 16-fold reduction of antibiotics MIC was observed. Conclusions: Our findings suggest that the essential oil of Centroceras clavulatum should be further appraised for its potential use in the management of multi-drug resistant microorganisms, with the purpose to restore the activity of standard antimicrobial drugs.pt_PT
dc.description.sponsorshipRSP-2021/56
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.4103/2221-1691.321129pt_PT
dc.identifier.eissn2221-1691
dc.identifier.urihttp://hdl.handle.net/10400.1/16928
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMedknow Publicationspt_PT
dc.relationCentre of Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMacroalgaept_PT
dc.subjectCentroceras clavulatum (C. Agardh)pt_PT
dc.subjectAntimicrobial potentialpt_PT
dc.subjectMicrobial drug resistancept_PT
dc.subjectMarine natural productspt_PT
dc.subjectSynergism with antibioticspt_PT
dc.titleIn vitro antimicrobial and synergistic effect of essential oil from the red macroalgae Centroceras clavulatum (C. Agardh) Montagne with conventional antibioticspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PRAXIS XXI/4179/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage414pt_PT
oaire.citation.titleAsian Pacific Journal of Tropical Biomedicinept_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamPRAXIS XXI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCustódio
person.givenNameLuísa
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryf9cfed0f-6b67-413e-988c-ac7397183471
relation.isProjectOfPublication7de31c2e-29dc-4102-acf9-62fae3a7f2b7
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