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Fusions of a carbohydrate binding module with the small cationic hexapeptide RWRWRW confer antimicrobial properties to cellulose-based materials

2022Journal article Restricted access
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dc.contributor.authorBarbosa, Mariana
dc.contributor.authorSimões, Hélvio
dc.contributor.authorPinto, Sandra N.
dc.contributor.authorMacedo, Ana S.
dc.contributor.authorFonte, Pedro
dc.contributor.authorPrazeres, D.Miguel F.
dc.date.accessioned2022-10-19T10:18:18Z
dc.date.available2022-10-19T10:18:18Z
dc.date.issued2022
dc.description.abstractThe emergence of antibiotic-resistant bacteria is a critical worldwide healthcare problem. In the specific case of wound care, new and effective alternatives to currently available solutions are urgently needed. Cellulose-based dressings, for example, could be made more attractive if rendered antimicrobial. This work proposes a new strategy to modify cellulose-based materials with the short antimicrobial hexapeptide MP196 (RWRWRW - NH 2 ) that relies on a biomolecular recognition approach based on carbohydrate binding modules (CBMs). Specifically, we focused on the modification of hydrogels, paper, and microfibrillated cellulose (MFC) with fusions of the CBM3 from Clostridium thermocellum ( C. thermocellum ) with derivatives of MP196. The fusions are prepared by promoting the formation of a disulfide bond between Cys-terminated derivatives of MP196 and a CBM3 that is pre-anchored in the materials. The CBM3MP196-modified materials displayed antibacterial activity against Escherichia coli ( E. coli ), Pseudomonas aeruginosa ( P. aeruginosa ) and Staphylococcus aureus ( S. aureus ) that was significantly higher when compared with the activity of materials prepared by physical adsorption of MP196. The biomolecular strategy provides a more favorable orientation, exposure, and distancing of the peptide from the matrix. This versatile concept provides a toolbox for the functionalization of cellulose materials of different origins and architectures with a broad choice in peptides. Functionalization under mild biological conditions avoids further purification steps, allowing for translational research and multiple applications as drug delivery systems, scaffolds for tissue engineering and biomaterials.pt_PT
dc.description.sponsorshipPPBI-POCI-01-0145-FEDER-022122
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.actbio.2022.02.042pt_PT
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/10400.1/18410
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationBiorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
dc.relationPRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
dc.relationInstitute for Bioengineering and Biosciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.subjectAntibacterialpt_PT
dc.subjectBiomaterialpt_PT
dc.subjectBiomolecular recognitionpt_PT
dc.subjectCarbohydrate-binding modulespt_PT
dc.subjectCellulosept_PT
dc.titleFusions of a carbohydrate binding module with the small cationic hexapeptide RWRWRW confer antimicrobial properties to cellulose-based materialspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
oaire.awardTitlePRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-CTM%2F30790%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/SAICTPAC%2F0019%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.endPage232pt_PT
oaire.citation.startPage216pt_PT
oaire.citation.titleActa Biomaterialiapt_PT
oaire.citation.volume143pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFonte
person.givenNamePedro
person.identifier.ciencia-id2410-123D-3385
person.identifier.orcid0000-0002-1115-9282
person.identifier.ridK-3215-2013
person.identifier.scopus-author-id55146900200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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