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Duplicated membrane estrogen receptors in the European sea bass (Dicentrarchus labrax): Phylogeny, expression and regulation throughout the reproductive cycle

2018Journal article Open Access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorAndrade, André
dc.contributor.authorEstêvão, M. Dulce
dc.contributor.authorAlvarado, M. Victoria
dc.contributor.authorFelip, Alicia
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-03-23T12:04:40Z
dc.date.available2020-03-23T12:04:40Z
dc.date.issued2018
dc.description.abstractThe numerous estrogen functions reported across vertebrates have been classically explained by their binding to specific transcription factors, the nuclear estrogen receptors (ERs). Rapid non-genomic estrogenic responses have also been recently identified in vertebrates including fish, which can be mediated by membrane receptors such as the G protein-coupled estrogen receptor (Gper). In this study, two genes for Gper, namely gpera and gperb, were identified in the genome of a teleost fish, the European sea bass. Phylogenetic analysis indicated they were most likely retained after the 3R teleost-specific whole genome duplication and raises questions about their function in male and female sea bass. Gpera expression was mainly restricted to brain and pituitary in both sexes while gperb had a widespread tissue distribution with higher expression levels in gill filaments, kidney and head kidney. Both receptors were detected in the hypothalamus and pituitary of both sexes and significant changes in gpers expression were observed throughout the annual reproductive season. In female pituitaries, gpera showed an overall increase in expression throughout the reproductive season while gperb levels remained constant. In the hypothalamus, gpera had a higher expression during vitellogenesis and decreased in fish entering the ovary maturation and ovulation stage, while gperb expression increased at the final atresia stage. In males, gpers expression was constant in the hypothalamus and pituitary throughout the reproductive cycle apart from the mid- to late testicular development stage transition when a significant up-regulation of gpera occurred in the pituitary. The differential sex, seasonal and subtype-specific expression patterns detected for the two novel gper genes in sea bass suggests they may have acquired different and/or complementary roles in mediating estrogens actions in fish, namely on the neuroendocrine control of reproduction.pt_PT
dc.description.sponsorshipAGL2009-11086; PROMETEOII/2014/051
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jsbmb.2017.12.019pt_PT
dc.identifier.issn0960-0760
dc.identifier.urihttp://hdl.handle.net/10400.1/13639
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmino acid sequencept_PT
dc.subjectAnimalspt_PT
dc.subjectBasspt_PT
dc.subjectMembrane proteinspt_PT
dc.subjectReceptorspt_PT
dc.subjectSequence homologypt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectPhylogenypt_PT
dc.subjectReproductionpt_PT
dc.subjectEstrogenpt_PT
dc.titleDuplicated membrane estrogen receptors in the European sea bass (Dicentrarchus labrax): Phylogeny, expression and regulation throughout the reproductive cyclept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.endPage242pt_PT
oaire.citation.startPage234pt_PT
oaire.citation.titleThe Journal of Steroid Biochemistry and Molecular Biologypt_PT
oaire.citation.volume178pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameEstêvão
person.familyNamePower
person.givenNamePatricia IS
person.givenNameAndré
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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