Publication
Omega-3 fatty acid desaturase genes isolated from Purslane (Portulaca oleracea L.): expression in different tissues and response to cold and wound stress
| dc.contributor.author | Teixeira, Monica | |
| dc.contributor.author | Carvalho, Isabel Saraiva de | |
| dc.contributor.author | Brodelius, Maria | |
| dc.date.accessioned | 2012-11-22T13:29:47Z | |
| dc.date.available | 2012-11-22T13:29:47Z | |
| dc.date.issued | 2010-02 | |
| dc.description.abstract | Two full-length cDNA clones PoleFAD7 and PoleFAD8, encoding plastidial omega-3 fatty acid desaturases were isolated from purslane (Portulaca oleracea). The encoded enzymes convert linoleic to alpha-linolenic acid (C18:3n-3). Three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains were identified in the sequence. Both genes were expressed in all analyzed tissues showing different levels of expression. PoleFAD7 was up-regulated by wounding but not by low temperature. PoleFAD8 was up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content were higher both, in wounded and intact leaves of plants exposed to low temperature. | por |
| dc.identifier.other | AUT: ICA01121; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/1853 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | ACS Publications | por |
| dc.relation.publisherversion | http://Www.Ncbi.Nlm.Nih.Gov/Pubmed/20070085 | por |
| dc.subject | Portulaca oleracea | por |
| dc.subject | Omega-3 fatty acid desaturase | por |
| dc.subject | FAD7 | por |
| dc.subject | FAD8 | por |
| dc.subject | Cold stress | por |
| dc.subject | Wounding | por |
| dc.title | Omega-3 fatty acid desaturase genes isolated from Purslane (Portulaca oleracea L.): expression in different tissues and response to cold and wound stress | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1877 | por |
| oaire.citation.startPage | 1870 | por |
| oaire.citation.title | Journal of Agricultural and Food Chemistry | por |
| oaire.citation.volume | 58 | por |
| person.familyName | Saraiva de Carvalho | |
| person.givenName | Isabel Maria Marques | |
| person.identifier.ciencia-id | 591A-3777-B036 | |
| person.identifier.orcid | 0000-0001-8057-3404 | |
| person.identifier.scopus-author-id | 35995183800 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | af26e659-578b-4e5f-a649-384cd5e4f8e3 | |
| relation.isAuthorOfPublication.latestForDiscovery | af26e659-578b-4e5f-a649-384cd5e4f8e3 |