Repository logo
 
Publication

The solution structure of a [3Fe-4S] ferredoxin: oxidised ferredoxin II from desulfovibrio gigas

1999Journal article Restricted access
Simple item page
dc.contributor.authorGoodfellow, B. J.
dc.contributor.authorMacedo, Anjos L.
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorMoura, Isabel
dc.contributor.authorWray, Victor
dc.contributor.authorMoura, José J. G.
dc.date.accessioned2014-06-17T15:31:37Z
dc.date.available2014-06-17T15:31:37Z
dc.date.issued1999
dc.date.updated2014-06-12T13:59:42Z
dc.description.abstractThe use of standard 2D NMR experiments in combination with 1D NOE experiments allowed the assignment of 51 of the 58 spin systems of oxidised [3Fe-4S] ferredoxin isolated from Desulfovibrio gigas. The NMR solution structure was determined using data from 1D NOE and 2D NOESY spectra, as distance constraints, and information from the X-ray structure for the spin systems not detected by NMR in torsion angle dynamics calculations to produce a family of 15 low target function structures. The quality of the NMR family, as judged by the backbone r.m.s.d. values, was good (0.80 Å), with the majority of f/c angles falling within the allowed region of the Ramachandran plot. A comparison with the X-ray structure indicated that the overall global fold is very similar in solution and in the solid state. The determination of the solution structure of ferredoxin II (FdII) in the oxidised state (FdIIox) opens the way for the determination of the solution structure of the redox intermediate state of FdII (FdIIint), for which no X-ray structure is available.por
dc.identifier.citationGoodfellow, B. J.; Macedo, A. L.; Rodrigues, Pedro; Moura, Isabel; Wray, Victor; Moura, José J. G. The solution structure of a [3Fe-4S] ferredoxin: oxidised ferredoxin II from Desulfovibrio gigas, Journal of Biological Inorganic Chemistry, 4, 4, 421-430, 1999.por
dc.identifier.issn0949-8257
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4348
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectNuclear magnetic resonancepor
dc.subjectSolution structurepor
dc.subjectFerredoxinpor
dc.subjectParamagnetic proteinpor
dc.subjectDesulfovibrio gigaspor
dc.titleThe solution structure of a [3Fe-4S] ferredoxin: oxidised ferredoxin II from desulfovibrio gigaspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage430por
oaire.citation.issue4por
oaire.citation.startPage421por
oaire.citation.titleJournal of Biological Inorganic Chemistrypor
oaire.citation.volume4por
person.familyNameRodrigues
person.givenNamePedro
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
The solution structure of a ferredoxin oxidised ferredoxin II.pdf
Size:
781.45 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)