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Diversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters

2009-05Journal article Open access
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dc.contributor.authorHardoim, C. C. P.
dc.contributor.authorCosta, R.
dc.contributor.authorAraujo, F. V.
dc.contributor.authorHajdu, E.
dc.contributor.authorPeixoto, R.
dc.contributor.authorLins, U.
dc.contributor.authorRosado, A. S.
dc.contributor.authorvan Elsas, J. D.
dc.date.accessioned2018-12-07T14:57:51Z
dc.date.available2018-12-07T14:57:51Z
dc.date.issued2009-05
dc.description.abstractMicroorganisms can account for up to 60% of the fresh weight of marine sponges. Marine sponges have been hypothesized to serve as accumulation spots of particular microbial communities, but it is unknown to what extent these communities are directed by the organism or the site or occur randomly. To address this question, we assessed the composition of specific bacterial communities associated with Aplysina fulva, one of the prevalent sponge species inhabiting Brazilian waters. Specimens of A. fulva and surrounding seawater were collected in triplicate in shallow water at two sites, Caboclo Island and Tartaruga beach, Buzios, Brazil. Total community DNA was extracted from the samples using "direct" and "indirect" approaches. 16S rRNA-based PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analyses of the total bacterial community and of specific bacterial groups-Pseudomonas and Actinobacteria-revealed that the structure of these assemblages in A. fulva differed drastically from that observed in seawater. The DNA extraction methodology and sampling site were determinative for the composition of actinobacterial communities in A. fulva. However, no such effects could be gleaned from total bacterial and Pseudomonas PCR-DGGE profiles. Bacterial 16S rRNA gene clone libraries constructed from directly and indirectly extracted DNA did not differ significantly with respect to diversity and composition. Altogether, the libraries encompassed 15 bacterial phyla and the candidate division TM7. Clone sequences affiliated with the Cyanobacteria, Chloroflexi, Gamma-and Alphaproteobacteria, Actinobacteria, Bacteroidetes, and Acidobacteria were, in this order, most abundant. The bacterial communities associated with the A. fulva specimens were distinct and differed from those described in studies of sponge-associated microbiota performed with other sponge species.
dc.description.sponsorshipSoil Biotechnology Foundation, Wageningen, The Netherlands; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1128/AEM.02101-08
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10400.1/11732
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Society for Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject16S Ribosomal-Rna
dc.subjectPolyketide synthase genes
dc.subjectPseudoceratina-Clavata
dc.subjectMicrobial community
dc.subjectAntimicrobial activities
dc.subjectCyanobacterial Symbiont
dc.subjectMediterranean sponges
dc.subjectPhylogenetic analysis
dc.subjectAnaerobic-bacteria
dc.subjectSoil
dc.titleDiversity of bacteria in the marine sponge Aplysina fulva in Brazilian coastal waters
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3343
oaire.citation.issue10
oaire.citation.startPage3331
oaire.citation.titleApplied and Environmental Microbiology
oaire.citation.volume75
person.familyNameda Silva Costa
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4495127c-16f2-4231-9fc1-b471c661036b
relation.isAuthorOfPublication.latestForDiscovery4495127c-16f2-4231-9fc1-b471c661036b

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