Identification of weakfish Cynoscion (Gill) in the Bay of Panama with RFLP markers

Vergara-Chen, Carlos; González-Wangüemert, Mercedes; Bermingham, Eldredge; D’Croz, L.

http://hdl.handle.net/10400.1/9037

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Authors

Vergara-Chen, Carlos

González-Wangüemert, Mercedes

Bermingham, Eldredge

D’Croz, L.

Abstract(s)

A molecular approach, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), was developed to identify the planktonic larvae of Cynoscion species. Species-specific mitochondrial DNA markers were developed using three restriction endonucleases (DdeI, HaeIII and HinfI). These markers permitted the accurate discrimination of the five Cynoscion species in the Bay of Panama.

Keywords

Animals Cytochromes b Fisheries Haplotypes Molecular sequence data Pacific Ocean Perciformes Polymerase chain reaction Species specificity Polymorphism Restriction fragment length Fish larvae Marine fish Mitochondrial DNA PCR-RFLP Species identification