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Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates

dc.contributor.authorS B Viegas, Carla
dc.contributor.authorSimes, D
dc.contributor.authorLaizé, Vincent
dc.contributor.authorWilliamson, M. K.
dc.contributor.authorPrice, P. A.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-07-07T13:40:21Z
dc.date.available2014-07-07T13:40:21Z
dc.date.issued2008-12-26
dc.date.updated2014-06-03T09:50:19Z
dc.description.abstractWe report the isolation of a novel vitamin K-dependent protein from the calcified cartilage of Adriatic sturgeon (Acipenser nacarii). This 10.2-kDa secreted protein contains 16 -carboxyglutamic acid (Gla) residues in its 74-residue sequence, the highest Gla percent of any known protein, and we have therefore termed it Gla-rich protein (GRP). GRP has a high charge density (36 negative 16 positive 20 net negative) yet is insoluble at neutral pH. GRP has orthologs in all taxonomic groups of vertebrates, and a paralog (GRP2) in bony fish; no GRP homolog was found in invertebrates. There is no significant sequence homology between GRP and the Gla-containing region of any presently known vitamin K-dependent protein. Forty-seven GRP sequences were obtained by a combination of cDNA cloning and comparative genomics: all 47 have a propeptide that contains a -carboxylase recognition site and a mature protein with 14 highly conserved Glu residues, each of them being carboxylated in sturgeon. The protein sequence of GRP is also highly conserved, with 78% identity between sturgeon and human GRP. Analysis of the corresponding gene structures suggests a highly constrained organization, particularly for exon 4, which encodes the core Gla domain. GRP mRNA is found in virtually all rat and sturgeon tissues examined, with the highest expression in cartilage. Cells expressing GRP include chondrocytes, chondroblasts, osteoblasts, and osteocytes. Because of its potential to bind calcium through Gla residues, we suggest that GRP may regulate calcium in the extracellular environment.por
dc.identifier.citationViegas, C.S.B.; Simes, D.C.; Laizé, V.; Williamson, M.K.; Price, P.A.; Cancela, M.L.Gla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebrates, Journal of Biological Chemistry, 283, 52, 36655-36, 2008.por
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M802761200
dc.identifier.issn0021-9258
dc.identifier.otherAUT: DSI00813; LCA00739
dc.identifier.urihttp://hdl.handle.net/10400.1/4703
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe American Society for Biochemistry and Molecular Biologypor
dc.relation.publisherversionhttp://www.jbc.org/content/283/52/36655.full.pdf+html?sid=71d7ab41-bfa5-485e-a426-72a0a9450c1cpor
dc.titleGla-rich protein (GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly conserved in vertebratespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F9077%2F2002/PT
oaire.citation.endPage36664por
oaire.citation.issue52por
oaire.citation.startPage36655por
oaire.citation.titleJournal of Biological Chemistrypor
oaire.citation.volume283por
oaire.fundingStreamSFRH
person.familyNameViegas
person.familyNameSimes
person.familyNameLaizé
person.familyNameCancela
person.givenNameCarla
person.givenNameDina
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridN-6695-2014
person.identifier.ridN-2789-2014
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
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relation.isProjectOfPublication4258be9b-5187-4751-a8ab-bffbb79ce031
relation.isProjectOfPublication.latestForDiscovery4258be9b-5187-4751-a8ab-bffbb79ce031

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