Publication
In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes
| dc.contributor.author | Reis, Diana | |
| dc.contributor.author | Acosta, N. G. | |
| dc.contributor.author | Almansa, E. | |
| dc.contributor.author | Navarro, J. C. | |
| dc.contributor.author | Tocher, D. R. | |
| dc.contributor.author | Monroig, O. | |
| dc.contributor.author | Andrade, José Pedro | |
| dc.contributor.author | Sykes, António V. | |
| dc.contributor.author | Rodríguez, C. | |
| dc.date.accessioned | 2018-11-09T20:48:25Z | |
| dc.date.available | 2018-11-09T20:48:25Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The highmortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum albumin (BSA). A control treatmentwithout [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substratesweremainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine. | pt_PT |
| dc.description.sponsorship | AGL 2010-22120-CO3 | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | https://doi.org/10.1016/j.aquaculture.2014.05.016 | pt_PT |
| dc.identifier.issn | 0044-8486 | |
| dc.identifier.other | AUT: JAN00399; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/10926 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | FURTHER RESEARCH REGARDING THE DEVELOPMENT OF CUTTLEFISH SEPIA OFFICINALIS AQUACULTURE TECHNOLOGY | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Common octopus | pt_PT |
| dc.subject | Lipid metabolism | pt_PT |
| dc.subject | Octopus vulgaris hatchlings | pt_PT |
| dc.subject | Radiolabeled substrates | pt_PT |
| dc.subject | Unsaturated fatty acids | pt_PT |
| dc.title | In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | FURTHER RESEARCH REGARDING THE DEVELOPMENT OF CUTTLEFISH SEPIA OFFICINALIS AQUACULTURE TECHNOLOGY | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F36100%2F2007/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F76863%2F2011/PT | |
| oaire.citation.endPage | 33 | pt_PT |
| oaire.citation.startPage | 28 | pt_PT |
| oaire.citation.title | Aquaculture | pt_PT |
| oaire.citation.volume | 431 | pt_PT |
| oaire.fundingStream | SFRH | |
| person.familyName | Reis | |
| person.familyName | Andrade | |
| person.familyName | Sykes | |
| person.givenName | Diana | |
| person.givenName | Jose | |
| person.givenName | António | |
| person.identifier | 107454 | |
| person.identifier.ciencia-id | D514-1A21-F020 | |
| person.identifier.ciencia-id | 7510-6641-5A42 | |
| person.identifier.orcid | 0000-0002-1373-4818 | |
| person.identifier.orcid | 0000-0002-5482-9776 | |
| person.identifier.orcid | 0000-0002-5207-0612 | |
| person.identifier.rid | C-3609-2012 | |
| person.identifier.scopus-author-id | 55505799300 | |
| person.identifier.scopus-author-id | 7201722097 | |
| person.identifier.scopus-author-id | 7102626426 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |
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