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The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node

2013Journal article Open access
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dc.contributor.authorInacio, Jose Manuel
dc.contributor.authorMarques, Sara
dc.contributor.authorNakamura, Tetsuya
dc.contributor.authorShinohara, Kyosuke
dc.contributor.authorMeno, Chikara
dc.contributor.authorHamada, Hiroshi
dc.contributor.authorBelo, Jose Antonio
dc.date.accessioned2018-12-07T14:57:58Z
dc.date.available2018-12-07T14:57:58Z
dc.date.issued2013
dc.description.abstractThe determination of left-right body asymmetry in mouse embryos depends on the interplay of molecules In a highly sensitive structure, the node. Here, we show that the localization of Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, Cerl2 protein displays a nodal flow-dependent dynamic behavior that controls the activity of Nodal in the node, and the transmission of the laterality information to the left lateral plate mesoderm (LPM). Our results indicate that Cerl2 initially localizes and prevents the activation of Nodal genetic circuitry on the right side of the embryo, and later its right-to-left translocation shutdowns Nodal activity in the node. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the LPM. Simultaneous genetic removal of both Nodal node inhibitors, Cerl2 and Lefty1, sustains even longer and bilateral his LPM expression.
dc.description.sponsorshipF.C.T.; IBB/CBME, LA
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0060406
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11787
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLeft-right asymmetry
dc.subjectLeft-right axis
dc.subjectVertebrate embryo
dc.subjectKupffers vesicle
dc.subjectLateral plate
dc.subjectCerberus-like
dc.subjectFluid-flow
dc.subjectHeart
dc.subjectHeterotaxia
dc.subjectExpression
dc.titleThe dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.startPagee60406
oaire.citation.titlePLoS ONE
oaire.citation.volume8
person.familyNameInacio
person.familyNameMarques
person.familyNameBelo
person.givenNameJose M
person.givenNameSara
person.givenNameJosé A.
person.identifier.ciencia-idB810-3281-8D8C
person.identifier.ciencia-idBB17-0F5D-A4FB
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0002-9790-8335
person.identifier.orcid0000-0001-6784-5612
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridO-1455-2018
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6604046494
person.identifier.scopus-author-id6602141392
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationd1a81b33-a919-48ab-94b2-0b0418552245
relation.isAuthorOfPublication20dd7142-5b7a-465f-8943-a6ac33b91df1
relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery20dd7142-5b7a-465f-8943-a6ac33b91df1

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