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Dietary butyrate helps to restore the Intestinal status of a marine teleost (Sparus aurata) fed extreme diets low in fish meal and fish oil

2016-11Journal article Open Access
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dc.contributor.authorEstensoro, Itziar
dc.contributor.authorBallester-Lozano, Gabriel
dc.contributor.authorBenedito-Palos, Laura
dc.contributor.authorGrammes, Fabian
dc.contributor.authorMartos-Sitcha, Juan Antonio
dc.contributor.authorMydland, Liv-Torunn
dc.contributor.authorCalduch-Giner, Josep Alvar
dc.contributor.authorFuentes, Juan
dc.contributor.authorKaralazos, Vasileios
dc.contributor.authorOrtiz, Alvaro
dc.contributor.authorOverland, Margareth
dc.contributor.authorSitja-Bobadilla, Ariadna
dc.contributor.authorPerez-Sanchez, Jaume
dc.date.accessioned2017-04-07T15:55:44Z
dc.date.available2017-04-07T15:55:44Z
dc.date.issued2016-11
dc.description.abstractThere is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (FM) and fish oil (FO) in gilthead sea bream diet. Three experiments were conducted. In a first trial (T1), the best dose (0.4%) of sodium butyrate (BP-70 (R) NOREL) was chosen after a short (9 weeks) feeding period. In a second longer trial (T2) (8 months), four diets were used: a control diet containing 25% FM (T2-D1) and three experimental diets containing 5% FM (T2-D2, T2-D3, T2-D4). FO was the only added oil in D1, while a blend of plant oils replaced 58% and 84% of FO in T2-D2, and T2-D3 and T2-D4, respectively. The latter was supplemented with 0.4% BP-70. In a third trial (T3), two groups of fish were fed for 12 and 38 months with D1, D3 and D4 diets of T2. The effects of dietary changes were studied using histochemical, immunohistochemical, molecular and electrophysiological tools. The extreme diet (T2-D3) modified significantly the transcriptomic profile, especially at the anterior intestine, up-regulating the expression of inflammatory markers, in coincidence with a higher presence of granulocytes and lymphocytes in the submucosa, and changing genes involved in antioxidant defences, epithelial permeability and mucus production. Trans-epithelial electrical resistance (Rt) was also decreased (T3-D3). Most of these modifications were returned to control values with the addition of BP-70. None of the experimental diets modified the staining pattern of PCNA, FABP2 or ALPI. These results further confirm the potential of this additive to improve or reverse the detrimental effects of extreme fish diet formulations.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0166564
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/9205
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.relationAdvanced Research Initiatives for Nutrition & Aquaculture
dc.relationAQUAculture infrastructures for EXCELLence in European Fish research
dc.relationAQUAculture infrastructures for EXCELlence in European fish research towards 2020
dc.relation.isbasedonWOS:000389474000011
dc.titleDietary butyrate helps to restore the Intestinal status of a marine teleost (Sparus aurata) fed extreme diets low in fish meal and fish oil
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAdvanced Research Initiatives for Nutrition & Aquaculture
oaire.awardTitleAQUAculture infrastructures for EXCELLence in European Fish research
oaire.awardTitleAQUAculture infrastructures for EXCELlence in European fish research towards 2020
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/288925/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/262336/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/652831/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPagee0166564
oaire.citation.issue11
oaire.citation.startPagee0166564
oaire.citation.titlePLoS One
oaire.citation.volume11
oaire.fundingStreamFP7
oaire.fundingStreamFP7
oaire.fundingStreamH2020
oaire.fundingStream5876
person.familyNameFuentes
person.givenNameJuan
person.identifier392998
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0003-0430-8734
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryd354b937-b18c-4ca5-a843-04a04269d60f
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