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First molecular characterization of grapevine virus B (GVB) in portuguese grapevine cultivars and improvement of the RT-PCR detection assay

2016-12Journal article Restricted access
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dc.contributor.authorFonseca, Filomena
dc.contributor.authorFernandes Duarte, Vilma Vanessa
dc.contributor.authorSantos, Margarida Teixeira
dc.contributor.authorBrazão, João
dc.contributor.authorEiras-Dias, Eduardo
dc.date.accessioned2017-04-07T15:55:43Z
dc.date.available2017-04-07T15:55:43Z
dc.date.issued2016-12
dc.description.abstractThis work describes the first molecular characterization of grapevine virus B (GVB) in Portuguese grapevine cultivars. During a routine screening of 44 accessions in the National Collection of Grapevine Varieties (CAN PRT051), 17 were found infected with GVB in DAS-ELISA assays with commercial antibodies. However, only six of the corresponding isolates were successfully amplified using primer pairs described in the literature. The sequence variants (ORF4-3'UTR, 1147 nt) retrieved from these isolates segregated into two phylogenetic groups, which included sequences from complete genomes available in GenBank. The highly discrepant results obtained using serological and RT-PCR-based diagnostic tools led to the design of a primer pair for detection of GVB, which allowed the amplification of a 606-bp GVB-specific fragment from all DAS-ELISA-positive isolates and also revealed the existence of false negatives in the serological testing.
dc.identifier.doi10.1007/s00705-016-3024-3
dc.identifier.issn0304-8608
dc.identifier.urihttp://hdl.handle.net/10400.1/9201
dc.language.isoeng
dc.peerreviewedyes
dc.relationMolecular probing for effective strategies to control fanleaf and leafroll in grapevine
dc.relation.isbasedonWOS:000386068400025
dc.titleFirst molecular characterization of grapevine virus B (GVB) in portuguese grapevine cultivars and improvement of the RT-PCR detection assay
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular probing for effective strategies to control fanleaf and leafroll in grapevine
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F109859%2F2009/PT
oaire.citation.endPage3540
oaire.citation.issue12
oaire.citation.startPage3535
oaire.citation.titleArchives of Virology
oaire.citation.volume161
oaire.fundingStream3599-PPCDT
person.familyNameFonseca
person.familyNameFernandes Duarte
person.givenNameFilomena
person.givenNameVilma Vanessa
person.identifier2193357
person.identifier.ciencia-idF110-5D6E-33CB
person.identifier.orcid0000-0003-3824-0459
person.identifier.orcid0000-0002-3007-6167
person.identifier.scopus-author-id7004434992
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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