Microsatellite markers for the giant kelp macrocystis pyrifera

Alberto, F.; Whitmer, A.; Coelho, N. C.; Zippay, M.; E, Varela-Álvarez; Raimondi, P. T.; Reed, D. C.; Serrão, Ester

http://hdl.handle.net/10400.1/4040

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Abstract(s)

We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.

Keywords

Macrocystis pyrifera Giant kelp Microsatellites Macroalga Genetic diversity

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http://hdl.handle.net/10400.1/4040

Citation

Alberto, F.; Whitmer, A.; Coelho, N.C.; Zippay, M.; Varela-Alvarez, E.; Raimondi, P.T.; Reed, D.C.; Serrão, E.A.Microsatellite markers for the giant kelp Macrocystis pyrifera, Conservation Genetics, 10, 6, 1915-19, 2009.