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Exploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: In vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studies

2019-02Journal article Restricted
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dc.contributor.authorTrampetti, Francesca
dc.contributor.authorPereira, Catarina
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorCelaj, Odeta
dc.contributor.authorD'Abrosca, Brigida
dc.contributor.authorZengin, Gokhan
dc.contributor.authorMollica, Adriano
dc.contributor.authorStefanucci, Azzurra
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2020-07-24T10:52:50Z
dc.date.available2020-07-24T10:52:50Z
dc.date.issued2019-02
dc.description.abstractIn this study, ethyl acetate, acetone, ethanol and water extracts from flowers, stems and roots of Cistanche phelypaea (L.) Cout were appraised for radical scavenging activity (RSA) towards 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) and superoxide free radicals, and for metal chelating activities on iron and copper ions. The water extracts had the highest antioxidant activity, especially those from roots and flowers, and were further appraised for in vitro inhibition of enzymes implicated on the onset of human ailments, namely acetyl- (AChE) and butyrylcholinesterase (BuChE) for Alzheimer's disease, alpha-glucosidase and alpha-amylase for diabetes, and tyrosinase for skin hyper-pigmentation disorders. The extracts had a higher activity towards BuChE, and the roots extract had the highest capacity to inhibit tyrosinase. Samples showed a low capacity to inhibit carbohydrate hydrolysing enzymes, except for the root extract with a good inhibition on glucosidase. Samples were then characterized by NMR (1D and 2D): the main metabolites identified in the flowers extract were iridoid glycosides, in particular gluroside and bartsioside. In stems, phenylehanoid glycosides (PhGs) and iri doids were detected, especially acteoside. In roots were detected essentially PhGs, mainly echinacoside and tubuloside A. Docking studies were performed on the identified compounds. A favorable binding energy of tubuloside A to tyrosinase was calculated, and indicated this compound as a possible competitive inhibitor of alpha-glucosidase and tyrosinase. Our results suggest that C. phelypeae is a promising source of biologically-active compounds with health promoting properties for pharmaceutical and biomedical applications. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipFoundation for Science and Technology (FCT, Portugal)Portuguese Foundation for Science and Technology
dc.description.sponsorshipFCTPortuguese Foundation for Science and Technology [IF/00049/2012, SFRH/BD/94407/2013, SFRH/BD/116604/2016]
dc.identifier.doi10.1016/j.jpba.2018.11.053
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.urihttp://hdl.handle.net/10400.1/14411
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectPhenylethanoid glycosides
dc.subjectChemical-constituents
dc.subjectHerba
dc.subjectTyrosinase
dc.subjectTubulosa
dc.titleExploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: In vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studies
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94407%2F2013/PT
oaire.citation.endPage128
oaire.citation.startPage119
oaire.citation.titleJournal of Pharmaceutical and Biomedical Analysis
oaire.citation.volume165
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameTrampetti
person.familyNameGuerreiro Pereira
person.familyNameRodrigues
person.familyNameCustódio
person.givenNameFrancesca
person.givenNameCatarina Alexandra
person.givenNameMaria João
person.givenNameLuísa
person.identifierNmxO5SIAAAAJ
person.identifierR-004-VNG
person.identifier.ciencia-id4512-3435-689C
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-8702-6876
person.identifier.orcid0000-0002-1131-8773
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id57191039641
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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