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Electrochemical DNA sensor for detection of single nucleotide polymorphisms

2003Journal article Open access
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dc.contributor.authorMarques, L. P. J.
dc.contributor.authorCavaco, Isabel Maria Palma Antunes
dc.contributor.authorPinheiro, J. P.
dc.contributor.authorRibeiro, Vera
dc.contributor.authorFerreira, Guilherme
dc.date.accessioned2013-12-03T13:59:01Z
dc.date.available2013-12-03T13:59:01Z
dc.date.issued2003
dc.date.updated2013-11-26T16:49:41Z
dc.description.abstractIn recent years there has been an increased interest in using biosensors for the recognition and monitoring of molecule interactions. DNA sensors and gene chips are particularly relevant for directly applying the information gathered from the genome projects. In this work electrochemical techniques are used to develop methodologies to detect DNA polymorphisms in human genes using cytochrome P450 3A4 (CYP3A4) as a model gene. CYP3A4*1B oligonucleotides were immobilized on the surface of a gold electrode and hybridized with fully complementary oligonucleotide sequences as well as with mismatched sequences corresponding to the CYP3A4*1A reference sequence. The methodology developed is based on double-stranded DNA’s ability to transport charge along nucleotide stacking. The perturbation of the double helix pi-stack introduced by a mismatched nucleotide reduces electron flow and can be detected by measuring the attenuation of the charge transfer. The methodology developed could identify CYP3A4*1A homozygotes by the 5 μC charge attenuation observed when compared with DNA samples containing at least one CYP3A4*1B allele.por
dc.description.sponsorshipPOCTI/ESP/39589/01
dc.identifier.citationMarques, L. P. J.; Cavaco, I.; Pinheiro, J. P.; Ribeiro, V.; Ferreira, G. N. M. Electrochemical DNA sensor for detection of single nucleotide polymorphisms, Clinical Chemistry and Laboratory Medicine, 41, 4, 475-481, 2003.por
dc.identifier.issn1434-6621
dc.identifier.otherAUT: JPI01020; VMA01368; GFE01723;
dc.identifier.urihttp://hdl.handle.net/10400.1/3204
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWalter de Grueterpor
dc.subjectBiosensorspor
dc.subjectDNA sensorspor
dc.subjectGenotypingpor
dc.subjectSinglepor
dc.subjectElectrochemistrypor
dc.titleElectrochemical DNA sensor for detection of single nucleotide polymorphismspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage481por
oaire.citation.issue4por
oaire.citation.startPage475por
oaire.citation.titleClinical Chemistry and Laboratory Medicinepor
oaire.citation.volume41por
person.familyNamePinheiro
person.familyNameRibeiro
person.givenNameJosé Paulo
person.givenNameVera
person.identifier.ciencia-id3411-732A-B7FB
person.identifier.orcid0000-0001-7925-9733
person.identifier.orcid0000-0002-9697-7830
person.identifier.ridE-8402-2013
person.identifier.ridG-3783-2011
person.identifier.scopus-author-id35838299700
person.identifier.scopus-author-id7006349364
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5caedbac-d2b5-4a86-91a7-083bf83edda3
relation.isAuthorOfPublication3f188970-86bb-4815-afd8-e5e70f922fd9
relation.isAuthorOfPublication.latestForDiscovery5caedbac-d2b5-4a86-91a7-083bf83edda3

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