Repository logo
 
Publication

Optimized production of MLM Triacylglycerols catalyzed by immobilized heterologous rhizopus oryzae lipase

2012Journal article Restricted access
Simple item page
dc.contributor.authorNunes, Patrícia A.
dc.contributor.authorCabral, Paula Pires
dc.contributor.authorGuillen, M.
dc.contributor.authorValero, F.
dc.contributor.authorDias, S. Ferreira
dc.date.accessioned2016-02-03T16:15:08Z
dc.date.available2016-02-03T16:15:08Z
dc.date.issued2012
dc.description.abstractResponse surface methodology was used to model and optimize the acidolysis of virgin olive oil with caprylic (C8:0) or capric (C10:0) acids, aimed at the production of low caloric triacylglycerols (TAG) of MLM type, in solvent free media, catalyzed by the heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergit(A (R)) C. This lipase was produced in the methylotrophic yeast Pichia pastoris Mut(s) phenotype (experiments with C10:0) or a Mut(+) phenotype (experiments with C8:0), under different operational conditions. The r-ROL used in experiments with C10:0 presented a hydrolytic activity about 5 times of that presented by r-ROL used in acidolysis with C8:0. The experiments were carried out following a central composite rotatable design, as a function of the molar ratio (MR) medium chain fatty acid/TAG (1.6-4.4) and temperature (25-55 A degrees C). Convex surfaces described by second order polynomials as a function of MR and temperature were well fitted to fatty acid incorporation values. After 24-h reaction, the predicted maximum incorporation of caprylic (15.5 mol%) or capric (33.3 mol%) acids in olive oil occurs at 37 and 35 A degrees C, respectively, and at C8:0/TAG of 2.8:1 or C10:0/TAG of 3:1. These predicted optima were experimentally validated. Fermentation conditions used in r-ROL production highly affected hydrolytic activity and to a lesser extent interesterification activity.
dc.identifier.doihttps://dx.doi.org/10.1007/s11746-012-2027-9
dc.identifier.issn0003-021X
dc.identifier.otherAUT: PCA00486;
dc.identifier.urihttp://hdl.handle.net/10400.1/7586
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relation.isbasedonP-002-8DX
dc.titleOptimized production of MLM Triacylglycerols catalyzed by immobilized heterologous rhizopus oryzae lipase
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1295
oaire.citation.startPage1287
oaire.citation.titleJournal of the American Oil Chemists' Society
oaire.citation.volume89
person.familyNameNunes Cabrita
person.givenNamePatrícia
person.identifier.orcid0000-0002-7148-8950
person.identifier.scopus-author-id7006823084
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b0b5899-4a35-4405-acbe-a8f548457da1
relation.isAuthorOfPublication.latestForDiscovery4b0b5899-4a35-4405-acbe-a8f548457da1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Optimized Production of MLM Triacylglycerols Catalyzed by Immobilized Heterologous Rhizopus oryzae Lipase.pdf
Size:
409.54 KB
Format:
Adobe Portable Document Format
Download

Collections