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Marine oomycetes of the genus halophytophthora harbor viruses related to bunyaviruses

2020Journal article Open access
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dc.contributor.authorBotella, Leticia
dc.contributor.authorJanoušek, Josef
dc.contributor.authorMaia, Cristiana
dc.contributor.authorJung, Marilia Horta
dc.contributor.authorRaco, Milica
dc.contributor.authorJung, Thomas
dc.date.accessioned2020-09-15T08:48:28Z
dc.date.available2020-09-15T08:48:28Z
dc.date.issued2020
dc.description.abstractWe investigated the incidence of RNA viruses in a collection of Halophytophthora spp. from estuarine ecosystems in southern Portugal. The first approach to detect the presence of viruses was based on the occurrence of dsRNA, typically considered as a viral molecule in plants and fungi. Two dsRNA-banding patterns (∼7 and 9 kb) were observed in seven of 73 Halophytophthora isolates tested (9.6%). Consequently, two dsRNA-hosting isolates were chosen to perform stranded RNA sequencing for de novo virus sequence assembly. A total of eight putative novel virus species with genomic affinities to members of the order Bunyavirales were detected and their full-length RdRp gene characterized by RACE. Based on the direct partial amplification of their RdRp gene by RT-PCR multiple viral infections occur in both isolates selected. Likewise, the screening of those viruses in the whole collection of Halophytophthora isolates showed that their occurrence is limited to one single Halophytophthora species. To our knowledge, this is the first report demonstrating the presence of negative (-) ssRNA viruses in marine oomycetes.pt_PT
dc.description.sponsorshipProject Phytophthora Research Centre CZ.02.1.01/0.0/0.0/15_003/0000453pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fmicb.2020.01467pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.1/14718
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectdsRNApt_PT
dc.subjectMycoviruspt_PT
dc.subject(-) ssRNApt_PT
dc.subjectRdRppt_PT
dc.subjectMarine virologypt_PT
dc.subjectEstuariespt_PT
dc.titleMarine oomycetes of the genus halophytophthora harbor viruses related to bunyavirusespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1467pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume11pt_PT
person.familyNameMaia
person.familyNameJung
person.givenNameCristiana
person.givenNameThomas
person.identifier.ciencia-idBC15-6F7F-F84A
person.identifier.ciencia-id6A14-0727-EDCD
person.identifier.orcid0000-0002-8654-2905
person.identifier.orcid0000-0003-2034-0718
person.identifier.ridD-6946-2017
person.identifier.ridF-1665-2013
person.identifier.scopus-author-id56106673800
person.identifier.scopus-author-id7201389242
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione2c8b094-8efd-417a-8e7a-2713665f70df
relation.isAuthorOfPublication76dbda58-7585-45f6-82f3-0ffee7fa58b1
relation.isAuthorOfPublication.latestForDiscoverye2c8b094-8efd-417a-8e7a-2713665f70df

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