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Vasotocin and isotocin regulate aquaporin 1 function in the sea bream

2015-03Journal article Open access
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dc.contributor.authorMartos-Sitcha, Juan Antonio
dc.contributor.authorCampinho, Marco António
dc.contributor.authorMiguel Mancera, Juan
dc.contributor.authorMartinez-Rodriguez, Gonzalo
dc.contributor.authorFuentes, Juan
dc.date.accessioned2018-12-07T14:53:41Z
dc.date.available2018-12-07T14:53:41Z
dc.date.issued2015-03
dc.description.abstractAquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains little studied. Therefore, in this study we investigated the regulatory role of vasotocin (AVTR) and isotocin (ITR) receptors in Aqp1 paralog gene function in the teleost gilthead sea bream (Sparus aurata). The complete coding regions of Aqp1a, Aqp1b, AVTR V1a2-type, AVTR V2-type and ITR from sea bream were isolated. A Xenopus oocyte-swelling assay was used to functionally characterize AQP1 function and regulation by AVT and IT through their cognate receptors. Microinjection of oocytes with Aqp1b mRNA revealed regulation of water transport via PKA (IBMX+forskolin sensitive), whereas Aqp1a mRNA injection had the same effect via PKC signaling (PDBU sensitive). In the absence of expressed receptors, AVT and IT (10(-8) mol l(-1)) were unable to modify AQP1 function. AVT regulated AQP1a and AQP1b function only when the AVTR V2-type was co-expressed. IT regulated AQP1a function, but not AQP1b, only when ITR was present. Considering that Aqp1a and Aqp1b gene expression in the sea bream intestine is highly salinity dependent in vivo, our results in ovo demonstrate a regulatory role for AVT and IT in AQP1 function in the sea bream in the processing of intestinal fluid to achieve osmoregulation.
dc.description.sponsorshipMinistry of Education (Spain) [FPU AP2008-01194]; Portuguese National Science Foundation [SFRH/BPD/66808/2009]; Ministry of Science and Education, Spain [AGL2010-14876]; National Science Foundation (FCT, Portugal) [PTDC/MAR-BIO/3811/2012]
dc.identifier.doi10.1242/jeb.114546
dc.identifier.issn0022-0949
dc.identifier.issn1477-9145
dc.identifier.urihttp://hdl.handle.net/10400.1/11637
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany Of Biologists Ltd
dc.relationMOLECULAR AND CELLULAR EFFECTS OF IOXINIL AND DIETHYLSTILBESTROL ON ZEBRAFISH THYROCYTE DEVELOPMENT AND ADULT HOMEOSTASIS
dc.subjectCollecting Duct Cells
dc.subjectEel Anguilla-Anguilla
dc.subjectFlounder Platichthys-Flesus
dc.subjectWater Channel
dc.subjectArginine-Vasotocin
dc.subjectSparus-Aurata
dc.subjectSeawater Acclimation
dc.subjectMolecular-Cloning
dc.subjectXenopus Oocytes
dc.subjectMessenger-Rna
dc.titleVasotocin and isotocin regulate aquaporin 1 function in the sea bream
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMOLECULAR AND CELLULAR EFFECTS OF IOXINIL AND DIETHYLSTILBESTROL ON ZEBRAFISH THYROCYTE DEVELOPMENT AND ADULT HOMEOSTASIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F66808%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3811%2F2012/PT
oaire.citation.endPage693
oaire.citation.issue5
oaire.citation.startPage684
oaire.citation.titleJournal of Experimental Biology
oaire.citation.volume218
oaire.fundingStream3599-PPCDT
person.familyNameMartos-Sitcha
person.familyNameCampinho
person.familyNameFuentes
person.givenNameJuan Antonio
person.givenNameMarco António
person.givenNameJuan
person.identifier346093
person.identifier392998
person.identifier.ciencia-id0C13-13D6-35DF
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-0151-7250
person.identifier.orcid0000-0002-5238-0506
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridL-6140-2014
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id55217586200
person.identifier.scopus-author-id8938999600
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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