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Effect of trace minerals and B vitamins on the proliferation/cytotoxicity and mineralization of a Gilthead Seabream bone–derived cell line

2020Journal article Restricted access
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dc.contributor.authorViegas, Michael N.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorSalgado, Maria A.
dc.contributor.authorAguiar, Carla
dc.contributor.authorDias, Jorge
dc.date.accessioned2020-08-06T12:01:08Z
dc.date.available2020-08-06T12:01:08Z
dc.date.issued2020
dc.description.abstractTrace minerals and vitamins are known modulators of bone metabolism, and dietary optimization of these components may improve skeletal development and reduce the occurrence of skeleton deformities in farmed fish. As for larval stages, mineral and water-soluble vitamin nutrition requirements are lacking in research efforts and knowledge is scarce. An in vitro cell system developed from gilthead seabream vertebra and capable of mineralization was used to assess the effect of B vitamins (thiamin and pyridoxine) and trace minerals (copper, manganese, and zinc in a sulfated and chelated form) on cell proliferation and extracellular matrix (ECM) mineralization. Dependent on dose, inhibition of cellular proliferation and/or cytotoxic effects was observed for all nutrients tested and LD50 values were determined: copper, 67.4-69.5 ppm; manganese, 20.9-29.8 ppm; zinc, 37.1-42.8 ppm in sulfated and chelated form respectively; thiamin, 6273 ppm; pyridoxine, 14226 ppm. ECM mineralization was enhanced by mineral (dose and form dependent) and vitamin (dose dependent) supplementation, at non-toxic concentrations below the determined LD50s. This in vitro work confirmed the mineralogenic action of trace minerals and water-soluble vitamins and provided valuable insights for subsequent in vivo nutritional trials.pt_PT
dc.description.sponsorshipAgência financiadora Fundacao para a Ciencia e a Tecnologia PDE/BDE/113672/2015pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s12011-019-01939-5pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/14622
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectTrace mineralspt_PT
dc.subjectVitaminspt_PT
dc.subjectCell culturept_PT
dc.subjectMineralizationpt_PT
dc.subjectGilthead seabreampt_PT
dc.titleEffect of trace minerals and B vitamins on the proliferation/cytotoxicity and mineralization of a Gilthead Seabream bone–derived cell linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage638pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage629pt_PT
oaire.citation.titleBiological Trace Element Researchpt_PT
oaire.citation.volume196pt_PT
person.familyNameLaizé
person.givenNameVincent
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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