Publication
Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles
dc.contributor.author | Rodrigues, Susana | |
dc.contributor.author | Alves, Ana D. | |
dc.contributor.author | Cavaco, Joana S. | |
dc.contributor.author | Pontes, Jorge Filipe | |
dc.contributor.author | Guerreiro, Filipa | |
dc.contributor.author | Rosa Da Costa, Ana | |
dc.contributor.author | Buttini, Francesca | |
dc.contributor.author | Grenha, Ana | |
dc.date.accessioned | 2019-11-20T15:07:25Z | |
dc.date.available | 2019-11-20T15:07:25Z | |
dc.date.issued | 2017-08 | |
dc.description.abstract | Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments. Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard. As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy. This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy. Microparticles were produced by spray-drying, at LBG/INH/RFB mass ratio of 10/1/0.5. The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region. Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect. Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors. (C) 2017 Elsevier B.V. All rights reserved. | |
dc.description.sponsorship | National Portuguese funding through FCT - Fundacao para a Ciencia e a Tecnologia [PTDC/DTP-FTO/0094/2012, UID/BIM/04773/2013, UID/Multi/04326/2013, UID/QUI/00100/2013, PEst-OE/QUI/UI4023/2011] | |
dc.identifier.doi | 10.1016/j.ijpharm.2017.06.088 | |
dc.identifier.issn | 0378-5173 | |
dc.identifier.issn | 1873-3476 | |
dc.identifier.uri | http://hdl.handle.net/10400.1/13031 | |
dc.language.iso | eng | |
dc.peerreviewed | yes | |
dc.publisher | Elsevier | |
dc.relation | DEVELOPMENT OF POLYSACCHARIDE-BASED CARRIERS FOR PULMONARY TUBERCULOSIS THERAPY | |
dc.relation | Fighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy | |
dc.subject | Alveolar macrophages | |
dc.subject | Physicochemical properties | |
dc.subject | In-vitro | |
dc.subject | Nanoparticles | |
dc.subject | Powder | |
dc.subject | Formulations | |
dc.subject | Chitosan | |
dc.subject | Design | |
dc.subject | Inhalation | |
dc.subject | Deposition | |
dc.title | Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles | |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | DEVELOPMENT OF POLYSACCHARIDE-BASED CARRIERS FOR PULMONARY TUBERCULOSIS THERAPY | |
oaire.awardTitle | Fighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//PD%2FBD%2F52426%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0094%2F2012/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00100%2F2013/PT | |
oaire.citation.endPage | 441 | |
oaire.citation.issue | 01-fev | |
oaire.citation.startPage | 433 | |
oaire.citation.title | International Journal of Pharmaceutics | |
oaire.citation.volume | 529 | |
oaire.fundingStream | 3599-PPCDT | |
oaire.fundingStream | 5876 | |
oaire.fundingStream | 5876 | |
oaire.fundingStream | 5876 | |
person.familyName | Rodrigues | |
person.familyName | Pontes | |
person.familyName | Guerreiro | |
person.familyName | Rosa da Costa | |
person.familyName | Grenha | |
person.givenName | Susana | |
person.givenName | Jorge Filipe | |
person.givenName | Filipa | |
person.givenName | Ana M | |
person.givenName | Ana | |
person.identifier | 305029 | |
person.identifier.ciencia-id | 9D1A-6537-0383 | |
person.identifier.ciencia-id | CB16-5CBD-8B89 | |
person.identifier.ciencia-id | BF1D-7EFA-19BC | |
person.identifier.ciencia-id | A418-A85E-5DB1 | |
person.identifier.ciencia-id | 091C-0D58-7225 | |
person.identifier.orcid | 0000-0002-0329-4265 | |
person.identifier.orcid | 0000-0002-6447-0242 | |
person.identifier.orcid | 0000-0001-6224-1553 | |
person.identifier.orcid | 0000-0003-0225-9537 | |
person.identifier.orcid | 0000-0002-2136-1396 | |
person.identifier.rid | E-2165-2012 | |
person.identifier.rid | H-1392-2017 | |
person.identifier.scopus-author-id | 55125292100 | |
person.identifier.scopus-author-id | 57195033461 | |
person.identifier.scopus-author-id | 53986075100 | |
person.identifier.scopus-author-id | 8607930100 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | |
rcaap.type | article | |
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