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Global analysis of gene expression in mineralizing fish vertebra-derived cell lines: new insights into anti-mineralogenic effect of vanadate

2011Journal article Open access
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dc.contributor.authorTiago, Daniel
dc.contributor.authorLaizé, Vincent
dc.contributor.authorBargelloni, Luca
dc.contributor.authorFerraresso, Serena
dc.contributor.authorRomualdi, Chiara
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-12T10:44:48Z
dc.date.available2014-06-12T10:44:48Z
dc.date.issued2011
dc.date.updated2014-06-03T09:42:36Z
dc.description.abstractBackground Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic cell lines and a 4 × 44K Agilent oligo-array, were used to identify molecular determinants of in vitro mineralization and genes involved in anti-mineralogenic action of vanadate. Results Global analysis of gene expression identified 4,223 and 4,147 genes differentially expressed (fold change - FC > 1.5) during in vitro mineralization of VSa13 (pre-chondrocyte) and VSa16 (pre-osteoblast) cells, respectively. Comparative analysis indicated that nearly 45% of these genes are common to both cell lines and gene ontology (GO) classification is also similar for both cell types. Up-regulated genes (FC > 10) were mainly associated with transport, matrix/membrane, metabolism and signaling, while down-regulated genes were mainly associated with metabolism, calcium binding, transport and signaling. Analysis of gene expression in proliferative and mineralizing cells exposed to vanadate revealed 1,779 and 1,136 differentially expressed genes, respectively. Of these genes, 67 exhibited reverse patterns of expression upon vanadate treatment during proliferation or mineralization. Conclusions Comparative analysis of expression data from fish and data available in the literature for mammalian cell systems (bone-derived cells undergoing differentiation) indicate that the same type of genes, and in some cases the same orthologs, are involved in mechanisms of in vitro mineralization, suggesting their conservation throughout vertebrate evolution and across cell types. Array technology also allowed identification of genes differentially expressed upon exposure of fish cell lines to vanadate and likely involved in its anti-mineralogenic activity. Many were found to be unknown or they were never associated to bone homeostasis previously, thus providing a set of potential candidates whose study will likely bring insights into the complex mechanisms of tissue mineralization and bone formation.por
dc.identifier.citationTiago, Daniel M; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, M. Global analysis of gene expression in mineralizing fish vertebra-derived cell lines: new insights into anti-mineralogenic effect of vanadate, BMC Genomics, 12, 1, 310-310, 2011.por
dc.identifier.doihttp://dx.doi.org/ 10.1186/1471-2164-12-310
dc.identifier.issn1471-2164
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4277
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBioMed Centralpor
dc.subjectFish vertebra-derivedpor
dc.subjectAnti-mineralogenicpor
dc.titleGlobal analysis of gene expression in mineralizing fish vertebra-derived cell lines: new insights into anti-mineralogenic effect of vanadatepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F45034%2F2008/PT
oaire.citation.issue1por
oaire.citation.startPage310por
oaire.citation.titleBMC Genomicspor
oaire.citation.volume12por
oaire.fundingStreamSFRH
person.familyNameTiago
person.familyNameLaizé
person.familyNameCancela
person.givenNameDaniel
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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