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Integument structure and function in juvenile Xenopus laevis with disrupted thyroid balance

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dc.contributor.authorCarvalho, Edison Samir Mascarelhas
dc.contributor.authorFuentes, J.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T09:55:54Z
dc.date.available2014-10-24T09:55:54Z
dc.date.issued2011
dc.description.abstractThe skin is the largest organ in the body and is a barrier between the internal and external environment. The present study evaluates how PTU, a goitrogen, that is used to treat hyperthyroidism affects the structure and electrical properties of the frog (Xenopus laevis) skin. The results are considered in the context of the two-membrane model established in the seminal work of Ussing and collegues in the 1940s and 1950s. In vitro experiments with skin from Xenopus adults revealed that PTU can act directly on skin and causes a significant increase (p < 0.05, One-way ANOVA) in short circuit current (Isc) via an amiloride-insensitive mechanism. Juvenile Xenopus exposed to waterborne PTU (5 mg/L) had a significantly bigger and more active thyroid gland (p < 0.01, Student’s t-test) than control Xenopus. The bioelectric properties of skin taken from Xenopus juveniles treated with PTU in vivo had a lower Isc, (3.05 ± 0.4, n = 13) and Rt (288.2 ± 39.5) than skin from control Xenopus (Isc, 4.19 ± 1.14, n = 14; Rt, 343.3 ± 43.3). A histological assessment of skin from PTU treated Xenopus juveniles revealed the epidermis was significantly thicker (p < 0.01, Student’s t-test) and had a greater number of modified exocrine glands (p < 0.01, Student’s t-test) in the dermis compared to control skin. Modifications in skin structure are presumably the basis for its changed bioelectric properties and the study highlights a site of action for environmental chemicals which has been largely neglected.por
dc.description.sponsorshipThe work was supported by POCI 2010 and the European Social Funds attributed by the Portuguese National Science Foundation (FCT) to CCMAR. Resources were used from the project ‘‘Arrested development: The Molecular and Endocrine Basis of Flatfish Metamorphosis’’ (Q5RS-2002-01192) financed by the Commission of the European Communities.por
dc.identifier.citationEdison S.M. Carvalho, Juan Fuentes, Deborah Power, "Integument structure and function in juvenile Xenopus laevis with disrupted thyroid balance" in General and Comparative Endocrinology 174 (2011) 301–308.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2011.09.007
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5455
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEpithelial transportpor
dc.subjectPTUpor
dc.subjectShort circuit currentpor
dc.subjectThyroid glandpor
dc.subjectXenopus laevis skinpor
dc.titleIntegument structure and function in juvenile Xenopus laevis with disrupted thyroid balancepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage308por
oaire.citation.issue3por
oaire.citation.startPage301por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume174por
person.familyNameFuentes
person.familyNamePower
person.givenNameJuan
person.givenNameDeborah Mary
person.identifier392998
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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