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Changes in the concentration of organic acids in roots and leaves of carob-tree under Fe deficiency

2014Journal article Restricted access
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dc.contributor.authorGama, Florinda
dc.contributor.authorSaavedra, Teresa
dc.contributor.authorMiguel, Maria Graça
dc.contributor.authorAbadia, A.
dc.contributor.authorDe Varennes, A.
dc.date.accessioned2015-06-15T14:53:33Z
dc.date.available2015-06-15T14:53:33Z
dc.date.issued2014
dc.description.abstractSeveral fruit trees are able to cope with iron (Fe) deficiency when grown in calcareous soils in the Mediterranean region, although information regarding well adapted slow-growing species is scarce, and the mechanisms activated by these species are not described in the literature. A crucial issue related to tolerance is the need to transport Fe over relatively long distances inside the plant. To evaluate the possible role of organic acids in the movement of Fe in tolerant plants, we studied the concentration of low molecular weight organic acids in several organs of 1-year old carob plants grown for 55 days in nutrient solutions without Fe (0 mu M Fe) or with 1 mu M Fe and 10 mu M Fe. Roots, stems and leaves were harvested, and the biomass, Fe and organic acid contents quantified. Total leaf chlorophyll (Chl) was evaluated in young leaves over the experimental period and the activity of root ferric chelate-reductase (FC-R; EC 1.16.1.17) was determined after 35 days, when deficiency symptoms appeared. Iron chlorosis was observed only at the end of the experiment in plants grown in the absence of Fe, and these plants had a smaller DW of leaves and also significant greater activity of root FC-R. Iron deficiency (Fe0 and Fe1 treatments) induced significant changes in the concentrations of succinic, malic, citric and fumaric acids, which increased in roots, or in basal, middle and apical leaves. There were significant correlations between most organic acids (with the exceptions of 2-oxoglutaric and tartaric acids) and leaf Chl. Analysis of each type of leaf showed that more succinic and malic acids were present in young chlorotic leaves while the reverse was true for quinic acid. These changes in organic acids followed a root-to-foliage pathway that was similar in all leaf types and particularly evident in young chlorotic leaves. We hypothesised that it was associated with Fe transport from roots to aboveground tissues, as there were significant differences in Fe contents between treatments with and without Fe.
dc.identifier.doihttps://dx.doi.org/10.1071/FP13204
dc.identifier.issn1445-4408
dc.identifier.issn1445-4416
dc.identifier.otherAUT: MGM00322;
dc.identifier.urihttp://hdl.handle.net/10400.1/6332
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCSIRO Publising
dc.relation.isbasedonP-009-9S1
dc.titleChanges in the concentration of organic acids in roots and leaves of carob-tree under Fe deficiency
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage504
oaire.citation.startPage496
oaire.citation.titleFunctional Plant Biology
oaire.citation.volume41
person.familyNameGama
person.familyNameSaavedra
person.familyNameMiguel
person.givenNameFlorinda
person.givenNameTeresa
person.givenNameMaria da Graça
person.identifier.ciencia-idB61D-C9B5-8AE2
person.identifier.ciencia-id3F1F-53D6-1014
person.identifier.ciencia-id6110-6FE7-DF28
person.identifier.orcid0000-0001-6948-0055
person.identifier.orcid0000-0002-2125-6342
person.identifier.orcid0000-0003-2507-4228
person.identifier.scopus-author-id35770492700
person.identifier.scopus-author-id36467141800
person.identifier.scopus-author-id35562054500
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication0fee6d6e-bb58-4d2c-b289-ed401148196f
relation.isAuthorOfPublicationb61cfa99-52bb-401a-bdbb-084ff6afe23f
relation.isAuthorOfPublication2507350f-9e60-4d4a-a096-53e58d93549c
relation.isAuthorOfPublication.latestForDiscovery2507350f-9e60-4d4a-a096-53e58d93549c

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