Publication
Chemical composition, antioxidant and alpha-Glucosidase-Inhibiting activities of the aqueous and hydroethanolic extracts of Vaccinium myrtillus Leaves
| dc.contributor.author | Bljajic, Kristina | |
| dc.contributor.author | Petlevski, Roberta | |
| dc.contributor.author | Vujic, Lovorka | |
| dc.contributor.author | Cacic, Ana | |
| dc.contributor.author | Sostaric, Nina | |
| dc.contributor.author | Jablan, Jasna | |
| dc.contributor.author | de Carvalho, Isabel Saraiva | |
| dc.contributor.author | Koncic, Marijana Zovko | |
| dc.date.accessioned | 2018-12-07T14:53:48Z | |
| dc.date.available | 2018-12-07T14:53:48Z | |
| dc.date.issued | 2017-05 | |
| dc.description.abstract | Vaccinium myrtillus (bilberry) leaf is traditionally used in southeastern Europe for the treatment of diabetes. In the present study, the ability of bilberry leaf extracts to inhibit carbohydrate-hydrolyzing enzymes and restore glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress was investigated. A comprehensive analysis of the antioxidant activity of two bilberry leaf extracts was performed. The aqueous extract showed excellent total antioxidant and chelating activity. Its antioxidant activity in the beta-carotene-linoleic acid assay was very good, reaching the activity of the antioxidant standard BHA (93.4 +/- 2.3% vs. 95.1 +/- 2.4%, respectively). The hydroethanolic extract (ethanol/H2O, 8:2, v/v), on the other hand, was a better radical scavenger and Fe2+ reducing agent. Furthermore, the aqueous extract was able to efficiently increase glutathione concentration in Hep G2 cells subjected to glucose-induced oxidative stress and restore it to the levels observed in non-hyperglycaemic cells. The hydroethanolic extract strongly inhibited alpha-glucosidase, with the IC50 statistically equal to the antidiabetic drug acarbose (0.29 +/- 0.02 mg/mL vs. 0.50 +/- 0.01 mg/mL, respectively). Phytochemical analysis revealed the presence of quercetin and kaemferol derivatives, as well as chlorogenic and p-coumaric acid. The study results indicate that V. myrtillus leaf may have promising properties as a supporting therapy for diabetes. | |
| dc.description.sponsorship | University of Zagreb | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | |
| dc.identifier.doi | 10.3390/molecules22050703 | |
| dc.identifier.issn | 1420-3049 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/11692 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI Ag | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Induced diabetic-nephropathy | |
| dc.subject | Oxidative stress | |
| dc.subject | In-Vitro | |
| dc.subject | Glutathione | |
| dc.subject | L. | |
| dc.subject | Hepatotoxicity | |
| dc.subject | Apoptosis | |
| dc.subject | Diseases | |
| dc.subject | Injury | |
| dc.subject | Fruit | |
| dc.title | Chemical composition, antioxidant and alpha-Glucosidase-Inhibiting activities of the aqueous and hydroethanolic extracts of Vaccinium myrtillus Leaves | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 5 | |
| oaire.citation.startPage | 703 | |
| oaire.citation.title | Molecules | |
| oaire.citation.volume | 22 | |
| person.familyName | Saraiva de Carvalho | |
| person.givenName | Isabel Maria Marques | |
| person.identifier.ciencia-id | 591A-3777-B036 | |
| person.identifier.orcid | 0000-0001-8057-3404 | |
| person.identifier.scopus-author-id | 35995183800 | |
| rcaap.rights | openAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | af26e659-578b-4e5f-a649-384cd5e4f8e3 | |
| relation.isAuthorOfPublication.latestForDiscovery | af26e659-578b-4e5f-a649-384cd5e4f8e3 |
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