Publication
Clonagem do gene de matrix gla protein (MGP) da espécie xenopus leavis (Daudin, 1802)
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Advisor(s)
Abstract(s)
O presente estudo teve por objectivo principal a identificação e caracterização do gene matrix Gla protein (MGP), na espécie Xenopus laevis (Daudin, 1802).Para o efeito, efectuo-se um rastreio a um banco genómico, utilizando-se como sonda a cadeia de cDNA da MGP específica de Xenopus laevis. Pela purificação dos clones obtidos, isolou-se um fragmento genómico com 4,8 Kb de comprimento, o qual foi sequenciado 1106 bp da sua extremidade 5'. Este fragmento corresponde a parte do gene da proteína, nomeadamente à metade 3' do exão III e intrão 3. Dado o comprimento do clone de DNA genómico isolado, é possível que neste fragmento esteja igualmente localizado o exão IV, actualmente a ser analisado.
A Xenopus laevis genomic library was screened using a Xenopus MGP ODNA probe Which lead to the purification of a positive 19 Kd genomic clone. Within this clone we identified a 4,8 Kb EcORI fragment containing the internal EcORI sequence from exon III, intron 3 and presumably exon IV, which is still being characterized. Interestingly, the boundary between exon III and intron 3 is located exactly at the same position in both the human and the Xenopus gene while the size of intron 3 is apparently quite larger in Xenopus than in the human gene.
A Xenopus laevis genomic library was screened using a Xenopus MGP ODNA probe Which lead to the purification of a positive 19 Kd genomic clone. Within this clone we identified a 4,8 Kb EcORI fragment containing the internal EcORI sequence from exon III, intron 3 and presumably exon IV, which is still being characterized. Interestingly, the boundary between exon III and intron 3 is located exactly at the same position in both the human and the Xenopus gene while the size of intron 3 is apparently quite larger in Xenopus than in the human gene.