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Prolactin regulates luminal bicarbonate secretion in the intestine of the sea bream (Sparus aurata L.)

2012-11-01Journal article Open access
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dc.contributor.authorFerlazzo, A.
dc.contributor.authorCarvalho, Edison Samir Mascarelhas
dc.contributor.authorGregorio, Silvia
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorTischitta, F.
dc.contributor.authorFuentes, J.
dc.date.accessioned2014-10-24T14:02:08Z
dc.date.available2014-10-24T14:02:08Z
dc.date.issued2012-11-01
dc.description.abstractThe pituitary hormone prolactin is a pleiotropic endocrine factor that plays a major role in the regulation of ion balance in fish, with demonstrated actions mainly in the gills and kidney. The role of prolactin in intestinal ion transport remains little studied. In marine fish, which have high drinking rates, epithelial bicarbonate secretion in the intestine produces luminal carbonate aggregates believed to play a key role in water and ion homeostasis. The present study was designed to establish the putative role of prolactin in the regulation of intestinal bicarbonate secretion in a marine fish. Basolateral addition of prolactin to the anterior intestine of sea bream mounted in Ussing chambers caused a rapid (<20min) decrease of bicarbonate secretion measured by pH-stat. A clear inhibitory dose–response curve was obtained, with a maximal inhibition of 60–65% of basal bicarbonate secretion. The threshold concentration of prolactin for a significant effect on bicarbonate secretion was 10ngml–1, which is comparable with putative plasma levels in seawater fish. The effect of prolactin on apical bicarbonate secretion was independent of the generation route for bicarbonate, as shown in a preparation devoid of basolateral HCO3 –/CO2 buffer. Specific inhibitors of JAK2 (AG-490, 50mmoll–1), PI3K (LY-294002, 75mmoll–1) or MEK (U-012610, 10mmoll–1) caused a 50–70% reduction in the effect of prolactin on bicarbonate secretion, and demonstrated the involvement of prolactin receptors. In addition to rapid effects, prolactin has actions at the genomic level. Incubation of intestinal explants of anterior intestine of the sea bream in vitro for 3h demonstrated a specific effect of prolactin on the expression of the Slc4a4A Na+–HCO3– co-transporter, but not on the Slc26a6A or Slc26a3B Cl–/HCO3 – exchanger. We propose a new role for prolactin in the regulation of bicarbonate secretion, an essential function for ion/water homeostasis in the intestine of marine fish.por
dc.description.sponsorshipThis research was funded by the Portuguese National Science and Technology Foundation, project PTDC/MAR/104008/2008 (Ministry of Science and Higher Education, Portugal and European Social Funds) awarded to J.F.por
dc.identifier.citationA. Ferlazzo, E. S. M. Carvalho, S. F. Gregorio, D. M. Power, A. V. M. Canario, F. Trischitta and J. Fuentes, "Prolactin regulates luminal bicarbonate secretion in the intestine of the sea bream (Sparus aurata L.)" in The Journal of Experimental Biology 215, 3836-3844.por
dc.identifier.doihttp://dx.doi.or/10.1242/jeb.074906
dc.identifier.issn0022-0949
dc.identifier.otherAUT: DPO00386; ACA00258
dc.identifier.urihttp://hdl.handle.net/10400.1/5498
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCompany of Biologistspor
dc.relation.publisherversionhttp://jeb.biologists.org/content/215/21/3836.full.pdf+htmlpor
dc.subjectProlactinpor
dc.subjectIntestinepor
dc.subjectEpithelial transportpor
dc.subjectBicarbonate secretionpor
dc.subjectSea breampor
dc.subjectJAK2por
dc.subjectMEKpor
dc.subjectPI3Kpor
dc.subjectSLC4A4por
dc.subjectSLC26A6por
dc.subjectSLC26A3por
dc.titleProlactin regulates luminal bicarbonate secretion in the intestine of the sea bream (Sparus aurata L.)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3844por
oaire.citation.issue21por
oaire.citation.startPage3836por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume215por
person.familyNameGregorio
person.familyNamePower
person.familyNameCanario
person.familyNameFuentes
person.givenNameSilvia
person.givenNameDeborah Mary
person.givenNameAdelino
person.givenNameJuan
person.identifier1579837
person.identifier143624
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7201832526
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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