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Safe applications of ozone in tropical marine RAS: determining impacts of elevated total residual oxidants (TRO) on Stylophora pistillata and Xenia sp. holobionts

2026-02Artigo científico Acesso restrito
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dc.contributor.authorWeidlich, Sabine
dc.contributor.authorKeuter, Sabine
dc.contributor.authorAires, Tania
dc.contributor.authorEngelen, Aschwin
dc.contributor.authorKunzmann, Andreas
dc.date.accessioned2025-12-12T13:51:54Z
dc.date.available2025-12-12T13:51:54Z
dc.date.issued2026-02
dc.description.abstractOzone-aided treatment of seawater creates secondary oxidants, which can be toxic for aquaculture animals. We aimed to define safe limits of ozonation-derived total residual oxidants (TRO) for Stylophora pistillata and Xenia sp. by conducting a 96 h acute toxicity experiment. In a chronic exposure experiment we then determined the impacts of three sub-lethal levels of TRO on animal health over four weeks by monitoring respiration, net photosynthesis, photosynthetic efficiency, and three oxidative stress biomarkers (CAT, SOD, LPO). We further documented the changes in coral surface microbiomes exposed to elevated TRO with 16S rRNA amplicon gene sequence analysis. We showed that Xenia sp. is more susceptible to elevated TRO, with first mortalities occurring within 24 h at 0.188 mg L-1 and higher. In Stylophora, mortalities were observable within 24 h at 1.272 mg L− 1 and after 72 h at 0.353 mg L− 1 TRO. Sub-lethal TRO levels caused higher respiration and lower photosynthetic efficiency in Xenia, and increased catalase activity by 29–42 % in Stylophora and by 38–45 % in Xenia, while not impacting lipid peroxidation or superoxide dismutase activity. Microbiomes displayed TRO-induced changes in both species, with a decline in Saprospiraceae as a potential indicator for ozone-inflicted mucus layer degradation. Vibrionaceae fully disappeared from Stylophora pistillata microbiomes after 26 days of exposure to 0.131 mg L-1 TRO, suggesting ozonation as a potential tool to treat pathogens in coral aquaculture. Our study represents the first analysis of ozonation-induced impacts on coral holobionts, thereby providing a guideline for the safe application of ozone in coral cultivation.eng
dc.description.sponsorshipCEECINST/00114/2018
dc.identifier.doi10.1016/j.aquaculture.2025.743369
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/27951
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relation.ispartofAquaculture
dc.rights.uriN/A
dc.subjectCoral aquaculture
dc.subjectOxidative stress
dc.subjectOzone
dc.subjectWater treatment
dc.subjectRAS
dc.subjectMicrobiome
dc.titleSafe applications of ozone in tropical marine RAS: determining impacts of elevated total residual oxidants (TRO) on Stylophora pistillata and Xenia sp. holobiontseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.startPage743369
oaire.citation.titleAquaculture
oaire.citation.volume613
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAires
person.familyNameEngelen
person.givenNameTania
person.givenNameAschwin
person.identifier282868
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id911A-9A0C-744D
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0002-9579-9606
person.identifier.ridM-8306-2013
person.identifier.ridM-3432-2013
person.identifier.scopus-author-id6701622770
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationbb3e6904-8ea3-43e6-8c43-4979daafec9b
relation.isAuthorOfPublication33d5a223-f2c9-4c68-984f-9213f15a05b0
relation.isAuthorOfPublication.latestForDiscoverybb3e6904-8ea3-43e6-8c43-4979daafec9b
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relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
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