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Melatonin production improves Senegalese sole sperm motility at night, but fails as a supplement during cryopreservation

2024-12Journal article Open access
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dc.contributor.authorFélix de Azeredo Pinto e Melo, Francisca
dc.contributor.authorFerrão, Leonor
dc.contributor.authorGallego, Victor
dc.contributor.authorOliveira, Catarina
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2024-10-21T13:41:35Z
dc.date.available2024-10-21T13:41:35Z
dc.date.issued2024-12
dc.description.abstractMelatonin is a powerful antioxidant present in fish seminal plasma. This study aimed to understand melatonin's endogenous and exogenous effects on first-generation Senegalese sole sperm quality for sperm management applications. In the first experiment, samples were collected at mid-light (ML) and mid-dark (MD) daytimes, to evaluate the effects on sperm motility. In a second experiment, using confocal microscopy and melatonin-FITC, spermatozoa permeability to melatonin was evaluated and, after showing that it enters the nucleus and mitochondria by passive diffusion, exogenous melatonin toxicity and antioxidant potential during a cryopreservation assay were performed. The toxicity assay tested different melatonin concentrations (0.01, 0.1, 1, and 10 mM) and exposure times (3, 5, 15 and 30 min), and sperm motility parameters were measured (TM, PM, VCL, VSL, LIN) using CASA system. The best conditions (0.1 and 10 mM) were selected for the cryopreservation assay, and a set of post-thaw sperm quality analyses were performed (motility, viability, reactive oxygen species, lipid peroxidation, and DNA fragmentation). The motility analyzed at ML and MD showed significant differences in all parameters, mainly on velocities (VCL, VSL, VAP), that were significantly higher at MD. Supplemented melatonin did not influence spermatozoa motility, MDA content or DNA fragmentation, although a lower percentage of viable cells was obtained on the 10 mM treatment. Altogether, Senegalese sole spermatozoa motility was enhanced at night, putatively by endogenous melatonin through direct or indirect mechanisms, whereas supplemented melatonin did not confer extra protection during cryopreservation.eng
dc.description.sponsorshipCOVID/BD/153473/2023; t DL 57/2016/CP1361/CT0007; GA number: 101038049)
dc.identifier.doi10.1016/j.cryobiol.2024.104974
dc.identifier.issn0011-2240
dc.identifier.urihttp://hdl.handle.net/10400.1/26119
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofCryobiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleMelatonin production improves Senegalese sole sperm motility at night, but fails as a supplement during cryopreservationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of Senegalese sole sperm quality through the action of melatonin as an antioxidant
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148280%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage104794
oaire.citation.titleCryobiology
oaire.citation.volume117
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameFélix de Azeredo Pinto e Melo
person.familyNameFerrão
person.familyNameGallego
person.familyNameOliveira
person.familyNameCabrita
person.givenNameFrancisca
person.givenNameLeonor
person.givenNameVictor
person.givenNameCatarina
person.givenNameElsa
person.identifier.ciencia-idAC1C-FB89-E69C
person.identifier.ciencia-id8B13-2B8A-EDDE
person.identifier.ciencia-id0816-30C5-51AC
person.identifier.orcid0000-0001-9026-3522
person.identifier.orcid0000-0002-9733-0625
person.identifier.orcid0000-0002-6679-761X
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridK-9072-2017
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id33367852200
person.identifier.scopus-author-id35433190500
person.identifier.scopus-author-id26645697000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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