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The xenobiotic sensor PXR in a marine flatfish species (Solea senegalensis): Gene expression patterns and its regulation under different physiological conditions

2017-09Journal article Restricted access
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dc.contributor.authorMarques, Carlos
dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorGranadeiro, Luis
dc.contributor.authorTrindade, Marlene
dc.contributor.authorGavaia, Paulo
dc.contributor.authorLaizé, Vincent
dc.contributor.authorLeonor Cancela, M.
dc.contributor.authorFernandez, Ignacio
dc.date.accessioned2019-11-20T15:07:22Z
dc.date.available2019-11-20T15:07:22Z
dc.date.issued2017-09
dc.description.abstractThe pregnane X receptor (PXR) is a nuclear receptor belonging to the NR1I sub-family and a known master regulator of xenobiotic metabolism. New roles have been recently proposed in mammals through its activation by vitamin K (VK) such as regulation of glucose metabolism, bone homeostasis, reproduction, neuronal development and cognitive capacities. In marine fish species little is known about PXR and its potential roles. Here, expression patterns of pxr transcripts and conservation of protein domains were determined in the Senegalese sole (Solea senegalensis), a marine flatfish model species in aquatic ecotoxicology. In addition to a full coding sequence transcript (sspxrl), two variants lacking DNA and/or ligand binding domains (sspxr2 and sspxr3) were also identified. The expression of sspxrl during early development and in adult tissues was ubiquitous, but highest levels were observed in liver, intestine and skin. Expression was also detected by in situ hybridization in chondrocytes and cells from the granular and inner nuclear layers in three month old fish. Finally, sspxrl expression was shown to be differentially regulated under physiological conditions related with fasting, VK and warfarin metabolism. The present work provides new and basic knowledge regarding pxr sequence and expression patterns in a marine flatfish species to unveil the potential impact of xenobiotics on marine fish physiology, and will allow a better and more ecosystemic environmental risk assessment of different pollutants over the marine environments with the development of reporter assays using PXR sequences from evolutionary distantly marine species (such as vertebrate and invertebrate marine species). (C) 2017 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipFundo Europeu de Desenvolvimento Regional (FEDER)
dc.description.sponsorshipPrograma Operacional through research project-Universidade do Algarve (KLING project) [PROMAR 31-03-05-FEP0073]
dc.description.sponsorshipEuropean Commission (ERDF-COMPETE) [PEst-C/MAR/LA0015/2011]
dc.identifier.doi10.1016/j.marenvres.2017.07.021
dc.identifier.issn0141-1136
dc.identifier.issn1879-0291
dc.identifier.urihttp://hdl.handle.net/10400.1/13008
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Sci Ltd
dc.relationINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
dc.relationAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
dc.subjectPregnane-X-receptor
dc.subjectLigand-binding domain
dc.subjectNuclear receptor
dc.subjectTranscriptional regulation
dc.subjectFunctional-characterization
dc.subjectSignaling pathway
dc.subjectDrug-metabolism
dc.subjectIn-vivo
dc.subjectIdentification
dc.subjectZebrafish
dc.titleThe xenobiotic sensor PXR in a marine flatfish species (Solea senegalensis): Gene expression patterns and its regulation under different physiological conditions
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
oaire.awardTitleAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F82049%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F112992%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage199
oaire.citation.startPage187
oaire.citation.titleMarine Environmental Research
oaire.citation.volume130
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameRoberto
person.familyNameGranadeiro
person.familyNameTrindade
person.familyNameGavaia
person.familyNameLaizé
person.familyNameCancela
person.givenNameVânia
person.givenNameLuis
person.givenNameMarlene
person.givenNamePaulo
person.givenNameVincent
person.givenNameM. Leonor
person.identifier1230353
person.identifier.ciencia-id681E-40B7-331F
person.identifier.ciencia-id3212-D91A-8A9A
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0001-7072-3392
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridA-6470-2011
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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