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Publicação
The ovarian bone morphogenetic protein system in Oreochromis mossambicus
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Orientador(es)
Resumo(s)
The study of ovarian follicle growth in teleosts provides basic knowledge but it
may also find applications in the biomedical sciences, fisheries management,
environmental science and aquaculture. The processes regulating ovarian development
are still largely unknown, particularly for teleosts. Many of the genes involved and their
roles on the various processes associated with reproduction are yet to be found. Rising
experimental data support TGF-β superfamily members has key role players in
numerous features of follicle development. Here we have studied several TGF-β
superfamily members, namely several BMP subfamily ligands and receptors, and the
GDF subfamily member - GDF-9, to help understand their roles in the ovary physiology
of the teleost Oreochromis mossambicus. The characterization of those ligands and
receptors included cloning of their cDNA coding regions, the identification of protein
conserved domains and phylogenetic analysis. Several TGF-β members received special
attention due to their reported importance and relevance in mammalian ovarian follicle
development, namely BMP-2, BMP-4, BMP-6, BMP-7, BMP-15, GDF-9, BMPR-IA,
BMPR-IB and BMPR-II. mRNA expression analysis of mature gonads was carried out
on these TGF-β members. Because of their crucial participation in ovarian follicle
development, BMP-15, GDF-9 and BMPR-IB were further studied by northern blot and
in situ hybridization.
All BMP family members studied were detected in ovary and testis but, mainly
in ovary. BMP-15 and GDF-9 mRNAs were expressed abundantly in ovary compared to
other tissues. BMPR-IB, in addition to ovary, was expressed abundantly in brain. These
results further reinforce the essential role BMP family members in ovarian physiology.
Descrição
Dissertação de mest., Biotecnologia, Faculdade de Engenharia dos Recursos Naturais, Universidade do Algarve, 2008
Palavras-chave
Teses Biotecnologia Reprodução animal Desenvolvimento