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Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction

2023-10Journal article Open Access
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dc.contributor.authorGouveia, Marisol
dc.contributor.authorSchmidt, Cristine
dc.contributor.authorBasilio, Priscilla Gois
dc.contributor.authorAveiro, Susana
dc.contributor.authorDomingues, Pedro
dc.contributor.authorXia, Ke
dc.contributor.authorColón, Wilfredo
dc.contributor.authorVitorino, Rui
dc.contributor.authorFerreira, Rita
dc.contributor.authorSantos, Mário
dc.contributor.authorVieira, Sandra I.
dc.contributor.authorRibeiro, Fernando
dc.date.accessioned2023-11-28T10:05:34Z
dc.date.available2023-11-28T10:05:34Z
dc.date.issued2023-10
dc.description.abstractBackgroundHeart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.AimTo determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).MethodsEighteen patients with HFrEF (age: 63.4 +/- 6.5 years; LVEF: 33.4 +/- 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.ResultsThe exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 +/- 8.9 to 36.1 +/- 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 +/- 27.1 to 59.8 +/- 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with alpha-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 +/- 0.40 to 1.11 +/- 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 +/- 0.54 x 1011 to 1.51 +/- 0.59 x 1011, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.ConclusionExercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF.pt_PT
dc.description.sponsorshipFundacao para a Ciencia e a Tecnologia (FCT), POCI-01-0145-FEDER-030011, Fundação para a Ciências e a Tecnologia (FCT) BEX 0554/14 - 6pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11010-023-04884-zpt_PT
dc.identifier.eissn1573-4919
dc.identifier.issn1863-9704
dc.identifier.urihttp://hdl.handle.net/10400.1/20173
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationAveiro Institute of Biomedicine
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationResearch Center in Physical Activity , Health and Leisure
dc.relationUnit for Multidisciplinary Research in Biomedicine
dc.relationUnit for Multidisciplinary Research in Biomedicine
dc.relationAnalysis of protein aggregation in plasma of patients with heart failure with preserved ejection fraction to find novel therapeutic targets and monitor therapeutic interventions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHFrEFpt_PT
dc.subjectExercise trainingpt_PT
dc.subjectProtein aggregatespt_PT
dc.subjectProteomicspt_PT
dc.titleExercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAveiro Institute of Biomedicine
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleResearch Center in Physical Activity , Health and Leisure
oaire.awardTitleUnit for Multidisciplinary Research in Biomedicine
oaire.awardTitleUnit for Multidisciplinary Research in Biomedicine
oaire.awardTitleAnalysis of protein aggregation in plasma of patients with heart failure with preserved ejection fraction to find novel therapeutic targets and monitor therapeutic interventions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMEC-CAR%2F30011%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04501%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00617%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00215%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00215%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F128893%2F2017/PT
oaire.citation.titleMolecular and Cellular Biochemistrypt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
person.familyNameAveiro
person.givenNameSusana
person.identifier.ciencia-idD312-07B4-DA74
person.identifier.orcid0000-0002-6651-3226
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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