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Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

dc.contributor.authorMendoza, Hector
dc.contributor.authorDe la Jara, Adelina
dc.contributor.authorFreijanes, Karen
dc.contributor.authorCarmona, Laura
dc.contributor.authorRamos, Ana
dc.contributor.authorDuarte, Vanessa
dc.contributor.authorVarela, J.
dc.date.accessioned2014-07-23T16:51:09Z
dc.date.available2014-07-23T16:51:09Z
dc.date.issued2008
dc.description.abstractIn the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.por
dc.description.sponsorshipFCTpor
dc.description.sponsorshipOVERCAROTEN POCTI/MAR/15237/99; INTERREG 159-SAL - Atlantic Salt Ponds.
dc.identifier.citationMendoza, H., K. Freijanes, L. Carmona, A. Ramos, V. Duarte, J. Varela & A. de la Jara. (2008). Characterization of Dunaliella salina strains by flow cytometry: a new technique to select β-carotene hyperproducing strains. Electronic Journal of Biotechnology, 11 (4): doi:10.2225/vol11-issue4-fulltext-2.por
dc.identifier.issn0717-3458
dc.identifier.otherAUT: JVA01305
dc.identifier.urihttp://hdl.handle.net/10400.1/4836
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v11n4-2/637por
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFlow cytometrypor
dc.subjectCarotenoidspor
dc.subjectDunaliella salinapor
dc.subjectMicroalgaepor
dc.titleCharacterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strainspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleElectronic Journal of Biotechnologypor
oaire.citation.volume11por
person.familyNameRamos
person.familyNameVarela
person.givenNameAna
person.givenNameJoão
person.identifier.ciencia-id761B-F84D-0CF5
person.identifier.orcid0000-0003-1099-4005
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-3815-2013
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id55741416000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42936018-1328-4fd1-8adb-4d37441727ab
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscoveryd339a9e7-bc32-49c4-ba48-a17343d40092

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