Modelling and optimization of ethyl butyrate production catalysed by Rhizopus oryzae lipase

Grosso, Carla; Ferreira Dias, S.; Cabral, Paula Pires

http://hdl.handle.net/10400.1/7584

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Authors

Grosso, Carla

Ferreira Dias, S.

Cabral, Paula Pires

Abstract(s)

Response surface methodology was used to model and optimise the production of ethyl butyrate, catalysed by Rhizopus oryzae lipase immobilised in a hydrophilic polyurethane foam. Experiments were carried out following a central composite rotatable design, as a function of reaction temperature (T: 22-38 degrees C) initial butyric acid concentration (A: 0.031-0.619 M) and initial molar ratio ethanol/acid (MR; 0.257-2.443). After 48 h reaction time, the production of ethyl butyrate could be fitted to a surface described by a second-order polynomial model. A maximum ethyl butyrate concentration of 0.106 M, corresponding to 47% conversion into ester and a productivity of 2.21 mu mole/mL h, is expected at initial reaction conditions of T, A and MR of 33 degrees C, 0.225 M and 1.637, respectively. This maximum was experimentally confirmed. (c) 2012 Elsevier Ltd. All rights reserved.