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Silencing of FRO1 gene affects iron homeostasis and nutrient balance in tomato plants

2023Journal article Open access
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dc.contributor.authorGama, Florinda
dc.contributor.authorSaavedra, Teresa
dc.contributor.authorDandlen, Susana
dc.contributor.authorGarcía‐Caparrós, Pedro
dc.contributor.authorde Varennes, Amarilis
dc.contributor.authorNolasco, Gustavo
dc.contributor.authorCorreia, Pedro José
dc.contributor.authorPestana, Maribela
dc.date.accessioned2023-09-13T09:52:53Z
dc.date.available2023-09-13T09:52:53Z
dc.date.issued2023
dc.description.abstractBackgroundIron chlorosis is an abiotic stress of worldwide importance affecting several agronomic crops. It is important to understand how plants maintain nutrient homeostasis under Fe deficiency and recovery. AimsWe used the virus-induced gene silencing (VIGS) method to elucidate the role of the FRO1 gene in tomato plants and identify the impact on regulation of the root ferric-chelate reductase (FCR) activity and nutritional homeostasis. MethodsTomato plantlets cv. "Cherry" were transferred into half-strength Hoagland's nutrient solution containing 0.5 & mu;M of Fe (Fe0.5). In phase I, two treatments were established: control (Fe0.5) plants and VIGS-0.5 plants corresponding to plants with the FRO1 gene silenced. In phase II, plants from Fe0.5 and VIGS-0.5 were transferred to new nutrient solution and then grown for a further 14 days under 0 and 10 & mu;M of Fe (as 0.5 & mu;M would not be enough for the larger plants during phase II). Therefore, four treatments were imposed: Fe0, Fe10, VIGS-0, and VIGS-10. ResultsVIGS-0.5 plants had significantly lower chlorophyll (Chl) and root FCR activity compared to the respective non-silenced plants and retained more Cu and Zn in the roots at the expense of stems (Cu) or young leaves (Zn). Iron concentration in roots and stems decreased in FRO1 gene-silenced plants, compared to control plants, but the allocation to different organs was similar in both treatments. ConclusionsThere was a partial recovery of leaf Chl in the VIGS-10 plants and a higher concentration of Fe in all organs. In contrast, the allocation of Cu to roots decreased in the VIGS-10 plants.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jpln.202300071pt_PT
dc.identifier.issn1436-8730
dc.identifier.urihttp://hdl.handle.net/10400.1/19971
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationTransport of iron in plants: novel approaches to an old problem. Improved iron mobility and development of a new fertilizer based on grass-clipping extracts.
dc.relationEstratégias inovadoras para otimizar o uso do ferro em culturas hortícolas.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFerric-chelate reductasept_PT
dc.subjectIron deficiencypt_PT
dc.subjectIron homeostasispt_PT
dc.subjectNutrientspt_PT
dc.subjectVirus-induced gene silencingpt_PT
dc.titleSilencing of FRO1 gene affects iron homeostasis and nutrient balance in tomato plantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTransport of iron in plants: novel approaches to an old problem. Improved iron mobility and development of a new fertilizer based on grass-clipping extracts.
oaire.awardTitleEstratégias inovadoras para otimizar o uso do ferro em culturas hortícolas.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-PRO%2F3861%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F144764%2F2019/PT
oaire.citation.titleJournal of Plant Nutrition and Soil Sciencept_PT
oaire.fundingStream3599-PPCDT
person.familyNameGama
person.familyNameSaavedra
person.familyNameDandlen
person.familyNameNolasco
person.familyNameCorreia
person.familyNamePestana
person.givenNameFlorinda
person.givenNameTeresa
person.givenNameSusana Anahi
person.givenNameGustavo
person.givenNamePedro José
person.givenNameMaribela
person.identifier74793
person.identifier.ciencia-idB61D-C9B5-8AE2
person.identifier.ciencia-id3F1F-53D6-1014
person.identifier.ciencia-id6613-CD28-84BE
person.identifier.ciencia-id2E15-3704-0F55
person.identifier.ciencia-id791E-9EC5-55C9
person.identifier.orcid0000-0001-6948-0055
person.identifier.orcid0000-0002-2125-6342
person.identifier.orcid0000-0002-7025-6023
person.identifier.orcid0000-0003-4927-1189
person.identifier.orcid0000-0002-4319-1682
person.identifier.ridF-5741-2010
person.identifier.scopus-author-id35770492700
person.identifier.scopus-author-id36467141800
person.identifier.scopus-author-id6602625528
person.identifier.scopus-author-id7006210686
person.identifier.scopus-author-id7005146039
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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