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The supplementation of a microdiet with crystalline indispensable amino-acids affects muscle growth and the expression pattern of related genes in Senegalese sole (Solea senegalensis) larvae

2016-05Journal article Restricted access
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dc.contributor.authorCanada, Paula
dc.contributor.authorEngrola, S.
dc.contributor.authorMira, Sara
dc.contributor.authorTeodósio, Rita
dc.contributor.authorFernandes, Jorge M. O.
dc.contributor.authorSousa, Vera
dc.contributor.authorBarriga-Negra, Lúcia
dc.contributor.authorConceicao, Luis
dc.contributor.authorValente, Luisa M. P.
dc.date.accessioned2017-04-07T15:56:44Z
dc.date.available2017-04-07T15:56:44Z
dc.date.issued2016-05
dc.description.abstractThe full expression of growth potential in fish larvae largely depends on an efficient protein utilization, which requires that all the indispensable amino acids (IAAs) are provided at an optimum ratio. The effect of supplementing a practical microdiet with encapsulated crystalline-AA to correct possible IAA deficiencies was evaluated in Senegalese sole larvae. Two isonitrogenous and isoenergetic microdiets were formulated and processed to have approximately the same ingredients and proximate composition. The control diet (CTRL) was based on protein sources commonly used in the aquafeed industry. In the supplemented diet (SUP) 8% of an encapsulated fish protein hydrolysate was replaced by crystalline-AA in order to increase the dietary IAA levels. The microdiets were delivered from mouth-opening upon a co-feeding regime until 51 days after hatching (DAH). The larvae capacity to utilize protein was evaluated using an in vivo method of controlled tube-feeding during relevant stages throughout development: pre-metamorphosis (13 DAH); metamorphosis climax (19 DAH) and metamorphosis completion (25 DAH). Somatic growth was monitored during the whole trial. A possible effect on the regulation of muscle growth was evaluated through muscle cellularity and the expression of related genes (myf5, myod2, myogenin, mrf4, myhc and mstn1) at metamorphosis climax (19 DAH) and at a juvenile stage (51 DAH). The SUP diet had a negative impact on larvae somatic growth after the metamorphosis, even though it had no effect on the development of Senegalese sole larvae capacity to retain protein. Instead, changes in somatic growth may reflect alterations on muscle growth regulation, since muscle cellularity suggested delayed muscle development in the SUP group at 51 DAH. Transcript levels of key genes regulating myogenesis changed between groups, during the metamorphosis climax and at the 51 DAH. The group fed the SUP diet had lower dnmt3b mRNA levels compared to the CTRL group. Further studies are needed to ascertain whether this would possibly lead to an overall DNA hypomethylation in skeletal muscle. (C) 2016 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.aquaculture.2016.03.010
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/9508
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationEpigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE
dc.relationIMPROVING GROWTH POTENTIAL IN SENEGALESE SOLE THROUGH DIETARY PROTEIN: AN INTEGRATED APPROACH USING MUSCLE CELLULARITY, TRACER STUDIES AND GENE EXPRESSION
dc.relation.isbasedonWOS:000373375700019
dc.titleThe supplementation of a microdiet with crystalline indispensable amino-acids affects muscle growth and the expression pattern of related genes in Senegalese sole (Solea senegalensis) larvae
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEpigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE
oaire.awardTitleIMPROVING GROWTH POTENTIAL IN SENEGALESE SOLE THROUGH DIETARY PROTEIN: AN INTEGRATED APPROACH USING MUSCLE CELLULARITY, TRACER STUDIES AND GENE EXPRESSION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FMAR%2F110547%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F82149%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00482%2F2014%2FCP1217%2FCT0005/PT
oaire.citation.endPage169
oaire.citation.startPage158
oaire.citation.titleAquaculture
oaire.citation.volume458
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamInvestigador FCT
person.familyNameMorgado Canada
person.familyNameEngrola
person.familyNameMira
person.familyNameTeodósio
person.familyNameConceicao
person.givenNamePaula Alexandra
person.givenNameSofia
person.givenNameSara
person.givenNameRita
person.givenNameLuis
person.identifier237846
person.identifier1577554
person.identifier.ciencia-id1114-7DA5-615E
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-id1913-A912-4673
person.identifier.ciencia-id9219-5DB3-CD39
person.identifier.orcid0000-0002-6182-5677
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0001-8346-4449
person.identifier.orcid0000-0002-8648-1241
person.identifier.orcid0000-0001-6102-2068
person.identifier.ridA-2485-2012
person.identifier.ridH-6257-2011
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id6603417249
person.identifier.scopus-author-id35867417300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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