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In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings

2016-01Journal article Restricted access
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dc.contributor.authorReis, Diana
dc.contributor.authorRodriguez, C.
dc.contributor.authorAcosta, N. G.
dc.contributor.authorAlmansa, E.
dc.contributor.authorTocher, D. R.
dc.contributor.authorAndrade, José Pedro
dc.contributor.authorSykes, António V.
dc.date.accessioned2017-04-07T15:57:41Z
dc.date.available2017-04-07T15:57:41Z
dc.date.issued2016-01
dc.description.abstractThe transition of Sepia officinalis culture to industrial large scale has been hampered due to bottlenecks related to the limited knowledge on nutritional physiology of the species. Determination of the endogenous ability of S. officinalis hatchlings to metabolise unsaturated fatty acids (FA) may provide new insight on the capability of hatchlings to biosynthesise different FA, as well as lipid classes containing essential fatty acids (EFA). In the present study, cuttlefish hatchlings were incubated with [1-C-14] FA including C18 FA (18:1n-9, 18:2n-6, 18:3n-3) and long-chain polyunsaturated fatty acids (LC-PUFA) (20: 4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA)), which were added individually as potassium salts bound to bovine serumalbumin. As a result, it was possible to investigate the in vivo FAmetabolismof S. officinalis hatchlings by following the incorporation of specific [1-C-14] FA, which points to the suitability of this methodology to study lipid metabolism of newly hatched cephalopods. The majority of radioactivity incorporated was recovered esterified into polar lipids (PL). A pattern was detected, where [1-C-14] DHA, [1-C-14] C18 FA and their metabolic products were preferentially esterified into phosphatidylcholine, whereas [1-C-14] ARA and [1-C-14] EPA were mainly esterified into phosphatidylethanolamine. [1-C-14] C18 FA were the most transformed FA with several metabolites produced by elongation and possible desaturation being obtained. As a contrary the radioactivity incorporated into hatchling total lipid (TL) from supplemented [1-C-14] LC-PUFA only one elongation product was recovered from the three substrates, except for [1-C-14] ARA, where an unidentified product was also detected. The present in vivo results indicated that S. officinalis hatchlings may have capability for the first steps in the biosynthesis of ARA and EPA from 18: 2n-6 and 18: 3n-3, respectively, including the existence of a desaturase potentially involved. Nonetheless, considering the low desaturation rates detected, this process may not be sufficient to cover EFA demands during development of this species. Therefore, dietary ARA and EPA, as well as DHA, should be supplied during the hatchling stage of Sepia. While designing an inert diet, which ensures normal growth and development of this species during the hatchling stage, the C18 FA and LC-PUFA levels and ratios should be considered, since the esterification pattern detected in the present study suggested competition between these FA for esterification into specific lipid classes. Moreover, considering the observed esterification pattern of LC-PUFA into different lipid classes, it is likely that the DHA/EPA/ARA ratio, rather than DHA/EPA or EPA/ARA ratios, would be of great importance for S. officinalis hatchling development. (C) 2015 Elsevier B.V. All rights reserved.
dc.description.sponsorshipEU Directive 2010/63/EU for animal experimentation; projects OCTOPHYS (AGL 2010-22120-CO3) funded by the Spanish Government; SEPIATECH (31-03-05-FEP-2)
dc.identifier.doihttps://doi.org/10.1016/j.aquaculture.2015.07.012
dc.identifier.issn0044-8486
dc.identifier.otherAUT: JAN00399;
dc.identifier.urihttp://hdl.handle.net/10400.1/9789
dc.language.isoeng
dc.peerreviewedyes
dc.relationSEPIABREED - Improving the reproduction of the European cuttlefish, Sepia officinalis (Linnaeus, 1758) in captivity: a multidisciplinary approach
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.relation.isbasedonWOS:000364854000010
dc.subjectDHA
dc.subjectEPA
dc.subjectARA
dc.subjectRadiolabelled substrates
dc.subjectSepia officinalis hatchlings
dc.subjectUnsaturated fatty acids
dc.titleIn vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlings
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSEPIABREED - Improving the reproduction of the European cuttlefish, Sepia officinalis (Linnaeus, 1758) in captivity: a multidisciplinary approach
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F120876%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F76863%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.citation.endPage73
oaire.citation.startPage67
oaire.citation.titleAquaculture
oaire.citation.volume450
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStreamInvestigador FCT
person.familyNameReis
person.familyNameAndrade
person.familyNameSykes
person.givenNameDiana
person.givenNameJose
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-idD514-1A21-F020
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-1373-4818
person.identifier.orcid0000-0002-5482-9776
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id55505799300
person.identifier.scopus-author-id7201722097
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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