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Does a ghrelin stimulus during zebrafish embryonic stage modulate its performance on the long-term?

2019-02Journal article Restricted access
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dc.contributor.authorNavarro-Guillén, Carmen
dc.contributor.authorDias, Jorge
dc.contributor.authorRocha, Filipa
dc.contributor.authorCastanheira, M. F.
dc.contributor.authorMartins, Catarina I. M.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorGavaia, Paulo
dc.contributor.authorEngrola, Sofia
dc.date.accessioned2020-07-24T10:52:55Z
dc.date.available2020-07-24T10:52:55Z
dc.date.issued2019-02
dc.description.abstractMetabolic programming refers to the induction, deletion, or impaired development of a somatic structure or "setting" of a physiological system by an early life stimulus operated at a critical period during development. Ghrelin is the only known orexigenic gut hormone, is an acylated peptide that acts as an endogenous ligand specific for growth-hormone secretagogue-receptor. The aim of the present work was to evaluate if an in ovo ghrelin administration could positively influence the zebrafish performance in the long-term and to gain insight on the mechanisms associated to ghrelin regulation of food intake during the larval phase. Food intake, growth potential, protein metabolism, expression of target genes involved in ghrelin, feeding behaviour regulation and locomotor activity were assessed in zebrafish (Danio rerio) larvae at 25 days post-fertilization. Elevated levels of acylated ghrelin in zebrafish eggs did not result in increased growth or food intake. Differences in mRNA expression between larvae fasted for 16 h before and 1 h after feeding were found for igf1ra, gh1 and pomca. Moreover, ghrelin treated larvae showed higher swimming activity, indicating that the peptide may have an important role on foraging activity. The present study addressed for the first time the effects of an early stimulus of ghrelin during the embryonic stage of zebrafish, however, further studies are needed to clarify the metabolic pathways affected by the early stimulus as well as focus on the effects on metabolic regulation of energy balance through lipid and carbohydrate metabolism.
dc.description.sponsorshipFCT/MCTES (Portugal)Portuguese Foundation for Science and Technology [PTDC/CVT/102481/2008, UID/Multi/04326/2013]
dc.description.sponsorshipFCT - European Social Fund [IF/00482/2014/CP1217/CT0005]
dc.description.sponsorshipOperational Programme Human Potential
dc.description.sponsorshipFoundation for Science and Technology of Portugal (FCT)Portuguese Foundation for Science and Technology
dc.identifier.doi10.1016/j.cbpa.2018.10.019
dc.identifier.issn1095-6433
dc.identifier.issn1531-4332
dc.identifier.urihttp://hdl.handle.net/10400.1/14422
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectFood-intake
dc.subjectLocomotor-activity
dc.subjectLipid-metabolism
dc.subjectDanio-rerio
dc.subjectGrowth
dc.subjectPeptide
dc.subjectExpression
dc.subjectFish
dc.subjectStimulation
dc.subjectEndocrine
dc.titleDoes a ghrelin stimulus during zebrafish embryonic stage modulate its performance on the long-term?
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT%2F102481%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPage8
oaire.citation.startPage1
oaire.citation.titleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology
oaire.citation.volume228
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
person.familyNameNavarro-Guillén
person.familyNameRocha
person.familyNameLaizé
person.familyNameGavaia
person.familyNameEngrola
person.givenNameCarmen
person.givenNameFilipa
person.givenNameVincent
person.givenNamePaulo
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-id281B-5543-D0F3
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0001-5490-0328
person.identifier.orcid0000-0002-7531-9302
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridB-4463-2008
person.identifier.ridA-6470-2011
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id55901593400
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id8669620600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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